IDENTIFICATION OF BACTERIA DIRECT FROM BLOOD CULTURE

直接从血培养中鉴定细菌

• 批准号: 6510903
• 负责人: MYRON SASSER
• 金额: $ 33万
• 依托单位: MIDI, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-06 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6510903
• 关键词: bacterial disease; communicable disease diagnosis; computer program /software; diagnosis design /evaluation; fatty acids; gas chromatography; human subject; nosocomial infections; patient oriented research; rapid diagnosis; serology /serodiagnosis; technology /technique development

DESCRIPTION (Verbatim from Applicant's Abstract):Rapid identification of bacteria direct from a blood culture bottle would speed up patient treatment and recovery, thereby also greatly reducing hospital care costs. The current procedure of subculturing typically takes from 24 to 48 hours to obtain bacterial identifications. Gas chromatographic analysis of bacterial fatty acids has promise as a rapid and inexpensive identification technique if the system were more sensitive and if the impact of the blood fatty acids could be minimized. Splitless injection, purification of the air supply, decreased data rate collection and concentration of the sample provide an increase in sensitivity of more than 1,600,000X. Lysis of blood cells by saponin or detergents coupled with filtration greatly reduces contamination of the bacterial fatty acids by blood components. New algorithms show promise for reducing the impact of blood components and for naming the components of polymicrobic cultures. A novel technique for rapid derivatization of fatty acids shows promise of very rapid identification of the pathogens. As patient care costs exceed $1,000 per day, the new technique could produce great health care cost savings. Additionally, by allowing targeted treatment and decreased recovery times, the technique should reduce patient suffering and aid in reducing the number of nosocomial infections. PROPOSED COMMERCIAL APPLICATION: Not Available

描述（来自申请人摘要的逐字记录）： 直接从血培养瓶中取出细菌将加快病人的治疗速度 和恢复，从而也大大降低了医院护理费用。当前 继代培养程序通常需要24至48小时以获得 细菌鉴定细菌脂肪酸的气相色谱分析 酸有希望作为一种快速和廉价的鉴定技术， 系统更敏感，如果血液脂肪酸的影响可以 最小化不分流喷射，净化空气供应，减少数据 样品的收集速率和浓度提供了 灵敏度超过1，600，000倍。血细胞被皂苷或 洗涤剂与过滤相结合，大大减少了对 细菌脂肪酸的血液成分。新算法显示出 减少血液成分的影响， 多微生物培养一种快速衍生化脂肪酸的新方法 酸显示出非常快速鉴定病原体的希望。诸如患者 每天的护理费用超过1,000美元，这项新技术可以带来巨大的健康 节省护理费用。此外，通过允许有针对性的治疗和减少 恢复时间，该技术应减少患者的痛苦，并帮助 减少医院感染的数量。 拟定商业应用： 不可用