IDENTIFICATION OF BACTERIA DIRECT FROM BLOOD CULTURE

描述(逐字摘自申请者摘要)：快速识别 直接从血液培养瓶中提取细菌将加快患者的治疗速度 和康复，从而也大大降低了医院的护理成本。海流 继代培养过程通常需要24到48小时才能获得 细菌鉴定。细菌脂肪的气相色谱分析 ACID作为一种快速且廉价的识别技术很有希望，如果 系统更敏感，如果血液中脂肪酸的影响可以 最小化。无分流注射，空气供应净化，数据减少 样品的速率收集和浓缩提供了增加 灵敏度超过1,600,000倍。皂苷对血细胞的裂解作用 洗涤剂与过滤相结合大大减少了对 按血液成分分类的细菌脂肪酸。新的算法显示出前景 减少血液成分的影响，并为血液成分命名 多种微生物培养。脂肪快速衍生化新技术 酸显示了非常快速地识别病原体的希望。作为患者 每天的护理费用超过1000美元，这项新技术可能会产生巨大的健康 节省护理成本。此外，通过允许靶向治疗和减少 恢复时间，该技术应减少患者的痛苦和帮助 减少医院感染的数量。 建议的商业应用： 不可用

DESCRIPTION (Verbatim from Applicant's Abstract):Rapid identification of bacteria direct from a blood culture bottle would speed up patient treatment and recovery, thereby also greatly reducing hospital care costs. The current procedure of subculturing typically takes from 24 to 48 hours to obtain bacterial identifications. Gas chromatographic analysis of bacterial fatty acids has promise as a rapid and inexpensive identification technique if the system were more sensitive and if the impact of the blood fatty acids could be minimized. Splitless injection, purification of the air supply, decreased data rate collection and concentration of the sample provide an increase in sensitivity of more than 1,600,000X. Lysis of blood cells by saponin or detergents coupled with filtration greatly reduces contamination of the bacterial fatty acids by blood components. New algorithms show promise for reducing the impact of blood components and for naming the components of polymicrobic cultures. A novel technique for rapid derivatization of fatty acids shows promise of very rapid identification of the pathogens. As patient care costs exceed $1,000 per day, the new technique could produce great health care cost savings. Additionally, by allowing targeted treatment and decreased recovery times, the technique should reduce patient suffering and aid in reducing the number of nosocomial infections. PROPOSED COMMERCIAL APPLICATION: Not Available