NEW IR APPROACH FOR PROBING STABILITY OF PROTEIN DRUGS

探测蛋白质药物稳定性的新红外方法

• 批准号: 2024438
• 负责人: AICHUN DONG
• 金额: $ 9.97万
• 依托单位: UNIVERSITY OF NORTHERN COLORADO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2024438
• 关键词: analytical chemistry; analytical method; bioengineering /biomedical engineering; biotechnology; chemical stability; infrared spectrometry; interferometry; method development; molecular dynamics; protein denaturation; protein folding; protein structure function; structural biology; thermodynamics; thermostability

DESCRIPTION: Stability is a critical problem for commercializing biotechnologically produced proteins of therapeutic values. Dr. Dong proposes to contribute to the solution of this problem through the investigation of protein folding/unfolding by means of FT-IR monitoring of the amide I band. He will initially investigate the stability of model proteins as a function of guanidine hydrochloride. He plans to eventually expand the study of the unfolding model system to proteins with diverse secondary structures and higher degrees of thermal and chemical stabilities.

描述：稳定性是商业化的关键问题 生物技术生产的具有治疗价值的蛋白质。 董医生 建议通过以下方式促进解决这一问题： 通过FT-IR监测蛋白质折叠/去折叠的研究 酰胺I带。 他将首先研究模型的稳定性 蛋白质作为盐酸胍的功能。 他计划最终 将去折叠模型系统的研究扩展到具有不同结构的蛋白质 二级结构和更高程度的热稳定性和化学稳定性。

项目成果

Entrapping intermediates of thermal aggregation in alpha-helical proteins with low concentration of guanidine hydrochloride.

• DOI: 10.1074/jbc.m005374200
• 发表时间: 2000-09
• 期刊: The Journal of biological chemistry
• 作者: Aichun Dong;T. Randolph;John F. Carpenter