NEURO/IMMUNE INTERACTION IN OCULAR SYSTEM

眼系统中的神经/免疫相互作用

• 批准号: 2608667
• 负责人: H KATHLEEN DANNELLY
• 金额: $ 2.22万
• 依托单位: INDIANA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2608667
• 关键词: B lymphocyte; T lymphocyte; acetylcholinesterase; antibody formation; antigen antibody reaction; biological information processing; cell type; choline acetyltransferase; cytokine; enzyme activity; enzyme linked immunosorbent assay; eye; homeostasis; immunocytochemistry; laboratory mouse; model design /development; neuroimmunomodulation; parasympathetic nervous system; plaque assay; secretory immune system; statistics /biometry

DESCRIPTION: (Adapted from applicant's abstract) The objective of the proposed research is to develop a model system for studying interaction between the immune and nervous systems. Such interactions normally helps maintain both the physiological and behavioral components of homeostasis throughout the body. Previous studies in avian species indicated that parasympathetic and sympathetic nerve fibers serve a role in modulation of lymphocyte activity and consequently influence the secretory immune system. Whether manipulation of the immune system exerts reciprocal modulatory effects on the nervous system is currently unknown. This investigation aims to establish whether changes introduced through the immune system influence the parasympathetic division of the autonomic nervous system (ANS) with the eye of mouse as the experimental model. Preliminary studies indicate that modulation of cholinergic enzyme activity is induced primarily by T cell-dependent antigens. This may be due to the mobilization of T and/or accessory cells elaborating cytokines in the process. Subsequent experiments will explore effects of T cell- independent antigens on immunity and cholinergic neural activity. Mice will be immunized with antigen applied either topically on the eye or intraperitoneally. Thus, localized changes in the lacrimal gland and conjunctiva will be examined by immunohistochemistry to detect antibody production and lymphoid cell migration in conjunction with changes in acetylcholinesterase (AChE, EC 3.1.1.7) and choline acetyltransferase (ChAT, EC 2.3.1.6). B cells secreting specific antibody will be localized in mouse ocular tissues by plaque forming cell assay. The role of T and accessory cells will be evaluated un in vitro conditions by assessing the actions of cytokines on tissue cholinergic enzymes. Antibody production in circulation is assessed in individual mice by enzyme-linked immunosorbent assays. AChE and ChAT will be determined by radiometric assays. Brain and spleen serve as controls for ocular tissues, to distinguish the overall physiological changes that may accompany regional antigen application. This study will lend support to the hypothesis that secretory immunity and the ANS communicate bi- directionally to prevent host infections and maintain homeostasis.

描述：（改编自申请人的摘要） 提出的研究是开发一个模型系统，用于研究互动 免疫系统和神经系统之间的联系 这种互动通常有助于 维持体内平衡的生理和行为成分 在整个身体。 先前对鸟类的研究表明， 副交感神经和交感神经纤维在调节中起作用 淋巴细胞活性，从而影响分泌性免疫 系统 是否操纵免疫系统发挥相互调节 目前尚不清楚对神经系统的影响。 这次调查 旨在确定是否通过免疫系统引入的变化 影响自主神经系统的副交感神经分支 (ANS)以小鼠眼为实验模型。 初步 研究表明胆碱能酶活性的调节是 主要由T细胞依赖性抗原诱导。 这可能是由于 动员T细胞和/或辅助细胞，以产生细胞因子， 过程 随后的实验将探索T细胞的影响- 免疫和胆碱能神经活性的独立抗原。 小鼠 将用局部施用在眼睛上的抗原免疫， 腹腔注射 因此，泪腺的局部变化， 将通过免疫组织化学检查结膜以检测抗体 产生和淋巴细胞迁移，以及 乙酰胆碱酯酶（AChE，EC 3.1.1.7）和胆碱乙酰转移酶 (ChAT，EC 2.3.1.6）。 分泌特异性抗体的B细胞将被 通过噬斑形成细胞测定定位于小鼠眼组织中。 的 将在体外条件下评价T细胞和辅助细胞的作用 通过评估细胞因子对组织胆碱能酶的作用。 在个体小鼠中通过以下方法评估循环中的抗体产生： 酶联免疫吸附测定。 将测定AChE和ChAT 通过放射性分析。 脑和脾作为眼内 组织，以区分可能 伴随局部抗原应用。 这项研究将支持 假设分泌免疫和ANS沟通双- 定向预防宿主感染和维持体内平衡。

项目成果

Effects of T-lymphocyte-dependent and -independent immunity on cholinergic enzyme activity in mouse lacrimal gland.

T淋巴细胞依赖性和非依赖性免疫对小鼠泪腺胆碱能酶活性的影响。

• DOI: 10.1113/eph8602115
• 发表时间: 2001
• 期刊: Experimental physiology
• 影响因子: 2.7
• 作者: Sinha,K;Dannelly,HK;Ghosh,SK
• 通讯作者: Ghosh,SK