MECHANISMS OF HISTAMINES EFFECT ON LYMPHOKINE SECRETION

组胺影响淋巴因子分泌的机制

• 批准号: 2665628
• 负责人: MANZOOR M. KHAN
• 金额: $ 9.87万
• 依托单位: CREIGHTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2665628
• 关键词: biological signal transduction; cyclic AMP; enzyme activity; helper T lymphocyte; histamine; histamine receptor; immunoregulation; interferon gamma; interleukin 2; interleukin 5; laboratory mouse; lymphokines; protein biosynthesis; protein kinase A; spleen; tissue /cell culture

DESCRIPTION (Adapted from the Investigator's Abstract): Histamine is a primary mediator of anaphylaxis, a neurotransmitter, and is now also known to be an immune modulator. Histamine suppresses the production of IL-2 and IFNg from TH1 T-cells, but up-regulates IL-5 synthesis from TH2 cells. The molecular mechanisms by which histamine acts are not known, but some of its immunomodulatory effects are through an elevation of intracellular cAMP. There is also evidence that both the inhibitory and stimulatory activity of histamine on T-cells is mediated through protein kinase-A (PKA). This application seeks to further define the cellular mechanisms associated with the immunoregulatory role of histamine. Using cloned murine helper T-cells and mouse splenocytes, the goal of this study is to assess: 1) the involvement of PKA in T-cell cytokine secretion and mRNA expression; and 2) assess the role of histamine on protein tyrosine kinase activity in cloned T-cells and splenocytes. This study will help define the molecular mechanisms of histamine's action on the immune response.

描述（改编自研究者摘要）：组胺是一种