MECHANISMS OF HISTAMINES EFFECT ON LYMPHOKINE SECRETION

描述(改编自《调查者摘要》)：组胺是一种 过敏反应的主要媒介，一种神经递质，现在也已知 成为一种免疫调节剂。组胺抑制IL-2和IL-2的产生 从TH1 T细胞产生的IFNG，但上调TH2细胞合成的IL-5。这个 组胺作用的分子机制尚不清楚，但它的一些 免疫调节作用是通过提高细胞内cAMP来实现的。 也有证据表明，无论是抑制活性还是刺激活性 T细胞上的组胺是通过蛋白激酶A(PKA)介导的。这 应用程序寻求进一步定义与以下各项相关的细胞机制 组胺的免疫调节作用。利用克隆的小鼠辅助T细胞 和小鼠脾细胞，本研究的目的是评估：1) PKA参与T细胞细胞因子分泌和mRNA表达；2) 组胺对克隆细胞蛋白酪氨酸激酶活性的影响 T细胞和脾细胞。这项研究将有助于定义分子 组胺在免疫反应中的作用机制。

DESCRIPTION (Adapted from the Investigator's Abstract): Histamine is a primary mediator of anaphylaxis, a neurotransmitter, and is now also known to be an immune modulator. Histamine suppresses the production of IL-2 and IFNg from TH1 T-cells, but up-regulates IL-5 synthesis from TH2 cells. The molecular mechanisms by which histamine acts are not known, but some of its immunomodulatory effects are through an elevation of intracellular cAMP. There is also evidence that both the inhibitory and stimulatory activity of histamine on T-cells is mediated through protein kinase-A (PKA). This application seeks to further define the cellular mechanisms associated with the immunoregulatory role of histamine. Using cloned murine helper T-cells and mouse splenocytes, the goal of this study is to assess: 1) the involvement of PKA in T-cell cytokine secretion and mRNA expression; and 2) assess the role of histamine on protein tyrosine kinase activity in cloned T-cells and splenocytes. This study will help define the molecular mechanisms of histamine's action on the immune response.