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SELECTING NEW CODING SEQUENCES BY PCR WITH CDNA PRIMERS

SELECTING NEW CODING SEQUENCES BY PCR WITH CDNA PRIMERS
使用 CDNA 引物通过 PCR 选择新的编码序列
批准号:
2652107
负责人:
PUDUR JAGADEESWARAN
金额:
$28.51万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-09-01 至 2000-04-30

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中文摘要
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英文摘要
DESCRIPTION: (Adapted from the applicant's abstract) The purpose of this Phase II Small Business Technology Transfer project is to test the utility of a PCR chimeric cDNA selection (CDS) method for isolating unknown coding sequences from larger human genomic contigs. The CDS method is based on the principle of recombining two homologous DNAs. Thus the method will be used to generate chimeric human EST-zebrafish cDNA libraries having the human EST sequences at the 5'-ends and homologous zebrafish cDNA at the 3'-ends. These chimeric cDNAs could be used to map human ESTs in zebrafish mutant genomes. Another application of the CDS method will be to generate a human housekeeping cDNA library that should be useful in identifying tissue specific sequences. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE
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