Fish as Genetic Models for Aging

鱼类作为衰老的遗传模型

• 批准号: 6479801
• 负责人: PUDUR JAGADEESWARAN
• 金额: $ 7.29万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6479801
• 关键词: aging; alternatives to animals in research; genetic models; genetic techniques; histology; killifish; longevity; model design /development

Model systems such as Caenorhabditis elegans and Drosophila melanogaster have been used to study genetics of aging, which identified some important genes, associated with aging. However, these invertebrate models cannot identify longevity genes that are unique to vertebrates. We propose to use Cynolebias, a cyprinid fish, commonly known as annual fish because it has an average life span of one year. However, its utility as a genetic model has not been explored. The goal of this proposal is to establish that Cynolebias could be used as a vertebrate genetic model organism for aging studies. To accomplish this goal, in the first specific aim, we will determine life-span curves for the Cynolebias. In the second specific aim, we will establish the applicability of genetic methods to Cynolebias. We propose that the reagents and genetic methods employed in zebrafish such as chemical mutagenesis, in vitro fertilization, diploidization, identification of different strains and identification of linkage markers, can be adapted to Cynolebias since both these fish belong to the cyprinid form of fishes. The results of this proposal will generate sufficient background data for establishing Cynolebias as a model for aging research. These results will also serve as preliminary data for a future NIH R01 grant application focusing on screening of Cynolebias mutants for long life span.

秀丽隐杆线虫和果蝇等模型系统已被用于研究衰老遗传学，识别出一些与衰老相关的重要基因。然而，这些无脊椎动物模型无法识别脊椎动物特有的长寿基因。我们建议使用Cynolebias，一种鲤鱼，俗称一年生鱼，因为它的平均寿命为一年。然而，其作为遗传模型的实用性尚未得到探索。该提案的目标是确定 Cynolebias 可以用作衰老研究的脊椎动物遗传模型生物。为了实现这一目标，在第一个具体目标中，我们将确定 Cynolebias 的寿命曲线。在第二个具体目标中，我们将确定遗传方法对 Cynolebias 的适用性。我们建议斑马鱼所采用的试剂和遗传方法，如化学诱变、体外受精、二倍化、不同品系的鉴定和连锁标记的鉴定，可以适用于青鳅，因为这两种鱼都属于鲤科鱼类。该提案的结果将为将 Cynolebias 建立为衰老研究模型提供足够的背景数据。这些结果也将作为未来 NIH R01 拨款申请的初步数据，重点是筛选长寿命的 Cynolebias 突变体。

项目成果

Annual fish as a genetic model for aging.

• DOI: 10.1093/gerona/59.2.b101
• 发表时间: 2004-02
• 期刊: The journals of gerontology. Series A, Biological sciences and medical sciences
• 作者: Michael B. Herrera;P. Jagadeeswaran