SELECTING NEW CODING SEQUENCES BY PCR WITH CDNA PRIMERS

描述：（改编自申请人的摘要）的目的 小企业技术转让项目第二阶段将测试 PCR嵌合cDNA选择（CDS）方法用于分离 来自较大人类基因组重叠群的未知编码序列。 的CDS 该方法基于重组两个同源DNA的原理。 因此，该方法将用于产生嵌合人EST-斑马鱼 在5 ′-末端具有人EST序列的cDNA文库， 同源的斑马鱼cDNA在3 '端。这些嵌合cDNA可能是 用于在斑马鱼突变基因组中定位人类EST。 另一应用 CDS方法的一个重要步骤是产生人类管家cDNA文库 这应该有助于识别组织特异性序列。 拟议商业应用：不可用

DESCRIPTION: (Adapted from the applicant's abstract) The purpose of this Phase II Small Business Technology Transfer project is to test the utility of a PCR chimeric cDNA selection (CDS) method for isolating unknown coding sequences from larger human genomic contigs. The CDS method is based on the principle of recombining two homologous DNAs. Thus the method will be used to generate chimeric human EST-zebrafish cDNA libraries having the human EST sequences at the 5'-ends and homologous zebrafish cDNA at the 3'-ends. These chimeric cDNAs could be used to map human ESTs in zebrafish mutant genomes. Another application of the CDS method will be to generate a human housekeeping cDNA library that should be useful in identifying tissue specific sequences. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE