CONTROLLING ALLERGENS AND COSTS IN THE ANIMAL FACILITY

控制动物设施中的过敏原和成本

• 批准号: 2697346
• 负责人: Beverly J Paigen
• 金额: $ 36.34万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2697346
• 关键词: Helicobacter; Mus musculus; Pasteurella; Pneumocystis carinii; SCID mouse; air filtration; air sampling /monitoring; airborne allergen; ammonia; animal care; animal colony; athymic mouse; communicable disease control; enzyme linked immunosorbent assay; epizootiology; germ free condition; immediate hypersensitivity; occupational disease /disorder; occupational health /safety; polymerase chain reaction; postmortem; work site

DESCRIPTION (Adapted from the applicant's abstract): The overall goal of these studies is to establish cost-effective environmental conditions that support high standards of animal health and provide a comfortable, safe workplace. This application will test the animal health effects of changing animal husbandry procedures that are major sources of employee exposure to allergen. If these changes have no negative impact on animal health, instituting them should reduce allergen levels by more than 10-fold, and result in healthier workers and reduced animal care costs. By extrapolating the savings at TJL to all NIH-supported mouse care, the potential savings to NIH is $22 million a year. The first animal husbandry practice to be tested is housing animals in ventilated caging with cage pressure negative to the room and using enclosed negatively ventilated tables when changing cages. To measure allergen reduction, room and worker breathing zone allergen concentrations will be measured before and after the changes. Concern that cages with negative pressure might increase the transfer of pathogens among animals will be tested in an experimental animal room where infected mice will be isolated from other colonies. Transfer of Pneumocystis carinii from infected mice to pathogen-free immune-competent and immune-compromised mice will be monitored by several methods. The second animal husbandry change will be to reduce the frequency of cage changing from once every 7 days to once every 14 days. Allergen concentrations will be measured and compared for both changing schedules. The cage environment (ammonia, temperature, humidity) will be compared for each condition, and animal health will be assessed by reproductive performance, growth, immune system function, and by necropsy focused on the histology of the upper and lower respiratory systems of young mice. These studies will fill a number of gaps in scientific knowledge concerning the natural history of transmission of Pneumocystis among mice, and the harmful effects of ammonia in typical animal facilities where ammonia levels peak only for short times until bedding is changed or a litter is weaned. The use of mice naturally infected with P. carinii, Pasteurella pneumotropica and Helicobacter spp. will enable use of all three organisms in the negative pressure system.

描述(改编自申请人的摘要)：总体目标 这些研究是为了建立具有成本效益的环境条件， 支持高标准的动物健康，提供舒适、安全的 工作场所。这个应用程序将测试改变对动物健康的影响 畜牧业程序是员工接触的主要来源 过敏原。如果这些变化对动物健康没有负面影响， 制定它们应该会将过敏原水平降低10倍以上，并且 从而使工人更健康，并降低动物护理成本。通过外推 TJL为所有NIH支持的老鼠护理节省的成本，潜在的节省 美国国立卫生研究院的年收入为2200万美元。第一个被测试的畜牧业实践 把动物关在通风笼子里，笼子的压力是负的吗？ 更换笼子时，应使用封闭的、通风良好的桌子。 测量过敏原的减少、房间和工作人员呼吸区的过敏原 浓度将在变化前后进行测量。担心…… 带负压的笼子可能会增加病原体在 动物将在一个实验动物房间里进行测试，在那里感染了老鼠 将与其他殖民地隔离开来。卡氏肺孢子虫的转移 感染小鼠对无病原体免疫能力和免疫低下小鼠的影响 将通过几种方法进行监测。第二次畜牧业变革 将把笼子更换频率从每7天一次减少到 每14天一次。过敏原浓度将被测量和比较 对于这两个不断变化的时间表。笼子环境(氨、温度、 湿度)将针对每种情况进行比较，并将动物健康 通过生殖性能、生长发育、免疫系统功能和 尸检侧重于上呼吸道和下呼吸道的组织学。 幼小的老鼠。这些研究将填补科学研究领域的一些空白 对肺孢子虫自然传播史的认识 以及典型动物设施中氨的有害影响 氨水平只会在床上用品更换或一次 小猫断奶了。使用自然感染卡氏肺孢子虫的小鼠， 嗜肺巴氏杆菌和螺杆菌属。将允许使用所有 负压系统中的三种生物。