Phenotyping and In Silico Mapping of Quantitative Traits

数量性状的表型分析和计算机绘图

• 批准号: 7299597
• 负责人: Beverly J Paigen
• 金额: $ 16.08万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7299597
• 关键词: biochemical evolution; bioinformatics; computational biology; functional /structural genomics; genetic strain; laboratory mouse; phenotype; quantitative trait loci

Previous work by CGD faculty has established two critical properties of quantitative trait loci (QTL). First, they represent only a sub-set of the totality of genes functionally required for a complex trait such as the regulation of blood pressure or relative susceptibility to atherosclerosis. And second, the same sub-set of genes determine QTLs across mammalian species. QTLs for a given trait are often located at homologous chromosomal locations in human, mouse and rat. A probable explanation for these findings is that QTLs represent key regulatory functions whose role has been conserved over the course of mammalian evolution. Given that the QTLs for various phenotypes are responsible for most of the population variation on which evolution can act and that the identity of these QTL is an ancient property of mammals, genome dynamics predicts that the QTLs for fundamental physiological properties are likely to be genetically linked to promote the co-inheritance of favorable allelic combinations. The hypothesis of this project is that functional domains are a general feature of mammalian chromosomal organization and will be revealed by the clustering of QTLs affecting a phenotype. Rigorously testing this hypothesis requires that we efficiently locate QTLs at a resolution of a few Mb or less across the diversity of Mus subspecies.

CGD教师的先前工作已经建立了定量性状基因座（QTL）的两个关键特性。 首先，它们仅代表复杂性状所需的基因总数的子集 调节血压或对动脉粥样硬化的相对敏感性。其次，相同的子集 基因确定了跨哺乳动物物种的QTL。给定特征的QTL通常位于同源 人，小鼠和大鼠的染色体位置。这些发现的可能解释是QTL 代表关键的调节功能，其在哺乳动物进化过程中的作用是保守的。 鉴于各种表型的QTL是导致大多数人口变化的原因 哪种进化可以行动，这些QTL的身份是哺乳动物的古老特性，基因组 动力学预测，基本生理特性的QTL可能与遗传有关 促进有利的等位基因组合的共同发电。该项目的假设是功能性 域是哺乳动物染色体组织的一般特征，将通过聚类揭示 影响表型的QTL。严格检验该假设，要求我们有效地定位QTL 在MUS亚种的多样性中分辨出几个或更少的MB。