DEVELOP 3SSH/SAGE TECHNIQUE FOR GENE IDENTIFICATION

开发用于基因识别的 3SSH/SAGE 技术

• 批准号: 2739772
• 负责人: JANET D ROWLEY
• 金额: $ 7.4万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-24 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2739772
• 关键词: clinical research; complementary DNA; gene expression; human genetic material tag; human subject; leukemia; method development; molecular cloning; neoplasm /cancer genetics; nucleic acid sequence; polymerase chain reaction; subtraction hybridization

DESCRIPTION: (Applicant's Description) We propose to develop a technique we call 3' cDNA SSH/SAGE for the identification of differentially expressed genes at the genomic level through modification and integration of key elements from differential display (DD), suppressive subtraction hybridization (SSH) and serial analysis of gene expression (SAGE) techniques. This technique focuses on the 3' sequence of all of the expressed genes in a given cell population, removes most of the abundant templates and some portion of homologous templates, and provides a sequence tag for each expressed gene to use in a database search. The differentially expressed genes between two populations will be identified by comparison of the resulting gene expression profiles. This technique should require limited initial materials, significantly decrease the difficulty of the gene identification process, directly use the EST database for gene identification, and thus provides a simple tool for the comparative analysis of gene expression at the genomic level.

（申请人描述）我们建议开发一种技术