DEVELOP 3SSH/SAGE TECHNIQUE FOR GENE IDENTIFICATION

描述：(应聘者的描述)我们建议开发一种我们 Call 3‘cDNASSH/SAGE鉴定差异表达基因 通过对关键基因的修饰和整合在基因组水平上表达基因 差异显示(DD)中的元素，抑制减法 杂交(SSH)和基因表达序列分析(SAGE) 技巧。这项技术主要关注所有的3‘序列 在给定的细胞群体中表达的基因，去除了大部分丰富的 模板和部分同源模板，并提供了序列 在数据库搜索中使用的每个表达基因的标签。差异化的 两个群体之间表达的基因将通过比较 由此产生的基因表达谱。这项技术应该需要 有限的初始材料，大大降低了基因的难度 鉴定过程中，直接使用EST数据库进行基因 从而为比较分析提供了一种简单的工具 在基因组水平上的基因表达。

DESCRIPTION: (Applicant's Description) We propose to develop a technique we call 3' cDNA SSH/SAGE for the identification of differentially expressed genes at the genomic level through modification and integration of key elements from differential display (DD), suppressive subtraction hybridization (SSH) and serial analysis of gene expression (SAGE) techniques. This technique focuses on the 3' sequence of all of the expressed genes in a given cell population, removes most of the abundant templates and some portion of homologous templates, and provides a sequence tag for each expressed gene to use in a database search. The differentially expressed genes between two populations will be identified by comparison of the resulting gene expression profiles. This technique should require limited initial materials, significantly decrease the difficulty of the gene identification process, directly use the EST database for gene identification, and thus provides a simple tool for the comparative analysis of gene expression at the genomic level.