MOLECULAR AND BIOINFORMATIC IDENTIFICATION AND MAPPING
分子和生物信息学识别和绘图
基本信息
- 批准号:2749001
- 负责人:
- 金额:$ 14.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-08-15 至 2000-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Completion of the DNA sequence of the yeast genome has made accessible
a large number of questions about the organization and expression of
eukaryotic genomes. Important among these questions is defining a
complete minimum protein set necessary for eukaryotic cell growth and
regulation, key to understanding human cancer. A hallmark of the
eukaryotes is the abundant presence of introns, internal gene sequences
not found in the mature messenger RNAs (mRNAs) that specify the protein
coding capacity of the genome. The presence of introns clouds our
ability to see open reading frames in the genomic sequence. To
understand the complete coding capacity of the yeast genome, and of
other eukaryotic genomes, we must first be able to recognize introns
in the genomic sequence. With the complete sequence of the yeast genome
in hand, we have the opportunity to map the positions of all the
nuclear pre-mRNA introns in the yeast genome, and thus reveal its
protein coding capacity. At this writing 220 yeast introns are known
or predicted, but these have been identified in a biased, ad hoc
fashion. We have developed a powerful molecular approach to the direct
detection of introns in a manner not biased by the contents of the gene
in which it is embedded. Oligonucleotides complementary to the unique
lariat sequence formed during splicing ("branchmers") specifically
prime reverse transcription of lariat intron RNA. Mutations that
inactivate the lariat debranching enzyme cause dramatic accumulation
of intron RNA in yeast. Thus branchmer oligonucleotides will be used
to generate expressed intron probes. Our aims are (1) to create and
screen libraries of "expressed intron tag" clones derived from strains
of yeast that accumulate large-amounts of intron RNA. These clones will
be sequenced to generate a database of expressed intron sequences, (2)
to identify genomic sequences similar to known introns using informatic
approaches and test these for splicing potential in vivo, and (3) to
refine repeated applications of each approach until a complete set of
confirmed introns is mapped to the sequence of the genome. Finding all
the introns will be essential to the complete understanding of the
coding capacity of the genome.
酵母基因组的DNA序列的完成使人们可以获得
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Manuel Ares其他文献
Manuel Ares的其他文献
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{{ truncateString('Manuel Ares', 18)}}的其他基金
Structure, regulation, and evolution of the splicing machinery
熔接机械的结构、调节和演变
- 批准号:
10406517 - 财政年份:2022
- 资助金额:
$ 14.35万 - 项目类别:
Structure, regulation, and evolution of the splicing machinery
熔接机械的结构、调节和演变
- 批准号:
10622605 - 财政年份:2022
- 资助金额:
$ 14.35万 - 项目类别:
STRUCTURE/FUNCTION OF EUKARYOTIC RNASE III
真核 RNA 酶 III 的结构/功能
- 批准号:
2701806 - 财政年份:1997
- 资助金额:
$ 14.35万 - 项目类别:
STRUCTURE/FUNCTION OF EUKARYOTIC RNASE III
真核 RNA 酶 III 的结构/功能
- 批准号:
2910298 - 财政年份:1997
- 资助金额:
$ 14.35万 - 项目类别:
STRUCTURE/FUNCTION OF EUKARYOTIC RNASE III
真核 RNA 酶 III 的结构/功能
- 批准号:
2024112 - 财政年份:1997
- 资助金额:
$ 14.35万 - 项目类别:
MOLECULAR AND BIOINFORMATIC IDENTIFICATION AND MAPPING
分子和生物信息学识别和绘图
- 批准号:
2630784 - 财政年份:1997
- 资助金额:
$ 14.35万 - 项目类别:
STRUCTURE AND FUNCTION OF YEAST SMALL NUCLEAR RNPS
酵母小核RNPS的结构和功能
- 批准号:
3072924 - 财政年份:1989
- 资助金额:
$ 14.35万 - 项目类别:
相似海外基金
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0642025 - 财政年份:2007
- 资助金额:
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