NEURAL CONTROL OF SEA URCHIN SPINE LIGAMENTS

海胆脊柱韧带的神经控制

• 批准号: 6273598
• 负责人: JOSE DEL CASTILLO
• 金额: $ 1.03万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6273598
• 关键词: acetylcholine; actins; alternatives to animals in research; biomechanics; collagen; crosslink; electron microscopy; histochemistry /cytochemistry; immunofluorescence technique; ligaments; microfilaments; muscle contraction; muscle pharmacology; neuromuscular function; neuropharmacology; neuroregulation; neurotransmitters; proteoglycan; sea urchins

The main goal of the proposed research is to elucidate the nature and mechanism of the changes in the physical properties of the collagenous articular capsule, or ligament, that surrounds the spine-test joint of the primary spines of the sea urchin Eucidaris tribuloides. Under the influence of the nervous system the consistency of this ligament can change rapidly and reversibly between two extreme conditions: soft, i.e. extensible, and stiff, or inextensible. Acetylcholine (ACh) consistently stiffens the ligament and may be regarded as the putative catch-inducing neurotransmitter. Pharmacological techniques will be used to search for neurotransmitters or drugs which may induce simple softening and to describe their interactions with ACh. Electron microscopy of conventional preparations and of material stained with cuprolinic blue will be used to characterize cross-links between collagen fibrils and columns and to detect possible alterations in the arrangement of the proteoglycans associated with changes in mechanical properties. In addition, histochemical techniques, using fluorescent phalloidin, anti- actin antibodies and the decoration of actin filaments with heavy meromyosin, will be employed to test the possible presence of actin filaments in the ligament matrix.

本研究的主要目的是阐明 胶原蛋白的物理性质变化的机制 关节囊，或韧带，围绕脊柱测试关节 海胆Eucidaris tribuloides的初级刺。 下 神经系统的影响，该韧带的一致性可以 在两种极端条件之间快速可逆地变化：软，即 可伸展的、僵硬的或不可伸展的。 乙酰胆碱（ACh） 使韧带变硬，可能被视为假定的捕获诱导 神经传递素 药理学技术将用于寻找 神经递质或药物，可能会导致简单的软化， 描述它们与乙酰胆碱的相互作用。 电镜观察 常规制剂及铜灵蓝染色材料 将用于表征胶原纤维之间的交联， 列，并检测可能的变化，在安排的 与机械性能变化相关的蛋白聚糖。 在 此外，组织化学技术，使用荧光鬼笔环肽，抗- 肌动蛋白抗体和肌动蛋白丝的装饰与重 将被用来测试肌动蛋白的可能存在 韧带基质中的细丝。