HCG LH/CG RECEPTOR BINDING AND ACTIVATION

HCG LH/CG 受体结合和激活

• 批准号: 2905309
• 负责人: J DAVID PUETT
• 金额: $ 35.3万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2905309
• 关键词: Sf9 cell line; animal genetic material tag; biological signal transduction; chorionic gonadotropin; conformation; cyclic AMP; hormone receptor; human genetic material tag; luteinizing hormone; mutant; protein folding; protein sequence; protein structure function; receptor binding; secretion; site directed mutagenesis; structural biology; thermodynamics; thermostability

DESCRIPTION: (adapted from the applicant's abstract) The objective of this project is the elucidation of the structural determinants leading to specific high-affinity binding of hCG to its receptor and the mechanism of ligand-mediated receptor activation. There are two major aims, namely 1) Delineation of hormone and receptor binding determinants and 2) Elucidating the mechanism of receptor activation and transmembrane signaling. Site-directed mutagenesis of alpha subunit, hCG-beta, and LH/CG receptor will be used to test the hypothesis that discrete regions of hCG and of receptor are responsible for high-affinity binding. The investigations will exploit a hormone-receptor polypeptide fusion technique to construct a putative mini-gonadotropin. Receptor mutagenesis studies will be utilized to test the hypothesis that transmembrane helices 6 and 7 contain amino acid residues that are important in signaling but not ligand binding.

描述：（改编自申请人的摘要） 项目是阐明结构性决定因素， hCG与其受体的特异性高亲和力结合及其作用机制 配体介导的受体活化。 有两个主要目标，即1） 描述激素和受体结合决定簇和2）阐明 受体激活和跨膜信号传导的机制。 α亚基、hCG-β和LH/CG受体的定点突变 将被用来检验假设，即离散区域的hCG和 受体负责高亲和力结合。 调查将 利用酶-受体多肽融合技术构建了一个 推测的小促性腺激素 将利用受体诱变研究 为了检验跨膜螺旋6和7含有氨基酸的假设， 这些残基在信号传导中很重要，但在配体结合中不重要。