CONFORMATIONS AND MECHANISMS OF PITUITARY HORMONES

本申请旨在阐明某些构象和构象异构体， 促性腺激素、人绒毛膜促性腺激素和 促黄体生成素 物理化学研究将包括电子顺磁 共振、核磁共振和圆二色性。 的 机制研究将主要针对Leydig瘤的作用 细胞;这些研究包括促性腺激素碳水化合物在 引发生物反应，促性腺激素诱导的表征 蛋白质、雌激素的作用和促黄体激素的一种假定变体的作用 在肾脏中。 将对β-内啡肽进行理化研究 和衍生肽，以及阿片受体，其表现出 β-内啡肽特异性，将被表征。 最后，条例 将在转化的Leydig细胞中研究阿黑皮素原的表达。

This application is aimed at elucidation of certain conformational and mechanistic aspects of the gonadotropins, human choriogonadotropin and lutropin. The Physicochemical studies will include electron paramagnetic resonance, nuclear magnetic resonance and circular dichroism. The mechanistic studies will be directed mainly toward actions in Leydig tumor cells; these studies include the role of gonadotropin carbohydrate in eliciting a biological response, characterization of gonadotropin induced proteins, actions of estrogen and the action of a putative lutropin variant in kidneys. Physicochemical studies will be conducted on beta-endorphin and derived peptides, and the epsilon opiate receptor, which exhibits beta-endorphin specificity, will be characterized. Lastly, the regulation of pro-opiomelanocortin will be studied in transformed Leydig cells.