CONFORMATIONS AND MECHANISMS OF PITUITARY HORMONES

垂体激素的构象和机制

• 批准号: 3232391
• 负责人: J DAVID PUETT
• 金额: $ 32.69万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3232391
• 关键词: Leydig cells; adrenocorticotropic hormone; biotransformation; blood chemistry; brain metabolism; chemical structure function; chorionic gonadotropin; circular dichroism; conformation; cow; electron spin resonance spectroscopy; fibroblasts; glomerular filtration; glycopeptides; gonadotropin releasing factor; gonadotropins; growth factor; hormone inhibitor; hormone receptor; hormone regulation /control mechanism; human pregnant subject; human tissue; insulin; laboratory mouse; laboratory rat; liver metabolism; luteinizing hormone; mutant; neoplasm /cancer transplantation; neuropeptide receptor; nuclear magnetic resonance spectroscopy; opioid receptor; ornithine decarboxylase; pituitary gonadal axis; pituitary hormones; pregnancy; prolactin; protein structure; radioimmunoassay; sheep; somatotropin; steroid hormone metabolism; swine; testis neoplasms; tissue /cell culture; ultracentrifugation; urinalysis

This application is aimed at elucidation of certain conformational and mechanistic aspects of the gonadotropins, human choriogonadotropin and lutropin. The Physicochemical studies will include electron paramagnetic resonance, nuclear magnetic resonance and circular dichroism. The mechanistic studies will be directed mainly toward actions in Leydig tumor cells; these studies include the role of gonadotropin carbohydrate in eliciting a biological response, characterization of gonadotropin induced proteins, actions of estrogen and the action of a putative lutropin variant in kidneys. Physicochemical studies will be conducted on beta-endorphin and derived peptides, and the epsilon opiate receptor, which exhibits beta-endorphin specificity, will be characterized. Lastly, the regulation of pro-opiomelanocortin will be studied in transformed Leydig cells.

本申请旨在阐明某些构象和构象异构体， 促性腺激素、人绒毛膜促性腺激素和 促黄体生成素 物理化学研究将包括电子顺磁 共振、核磁共振和圆二色性。 的 机制研究将主要针对Leydig瘤的作用 细胞;这些研究包括促性腺激素碳水化合物在 引发生物反应，促性腺激素诱导的表征 蛋白质、雌激素的作用和促黄体激素的一种假定变体的作用 在肾脏中。 将对β-内啡肽进行理化研究 和衍生肽，以及阿片受体，其表现出 β-内啡肽特异性，将被表征。 最后，条例 将在转化的Leydig细胞中研究阿黑皮素原的表达。