MODE OF ACTION OF STEROID HORMONES

类固醇激素的作用方式

• 批准号: 2905021
• 负责人: ALLAN U MUNCK
• 金额: $ 25.16万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-30 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2905021
• 关键词: DNA footprinting; cell cycle; corticosteroid receptors; deoxyribonuclease I; estrogen receptors; hormone regulation /control mechanism; messenger RNA; mutant; neoplastic cell culture for noncancer research; nuclear runoff assay; phosphorylation; progesterone receptors; protein kinase; protein structure function; stress proteins; tissue /cell culture; transfection

DESCRIPTION (Adapted from the applicant's abstract): This proposal focuses on the relationship between glucocorticoid receptor (GR) function and dynamic transformations of GR by phosphorylation and association with heat shock proteins. Previous work by the principal investigator has identified in detail the sites at which GR is phosphorylated, and has shown cell-cycle dependent changes in GR phosphorylation and heat shock protein association/dissociation. The present proposal will extend these studies, addressing 1) the role of GR phosphorylation in GR function; 2) the role of cell-cycle dependent kinases in this phosphorylation; 3) the possibility that estrogen in progesterone receptors also traverse ATP-dependent cycles.

描述（改编自申请人摘要）：本提案 重点研究糖皮质激素受体（GR） GR通过磷酸化的功能和动态转化， 与热休克蛋白的关系校长以前的工作 研究者已经详细确定了GR发生的部位， 磷酸化，并显示GR的细胞周期依赖性变化 磷酸化和热休克蛋白缔合/解离。 的 目前的建议将扩展这些研究，解决1）GR的作用 GR功能中的磷酸化; 2）细胞周期依赖性的作用 3）雌激素在这种磷酸化中的可能性， 孕酮受体也穿越ATP依赖性周期。

项目成果

How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions.

• DOI: 10.1210/edrv.21.1.0389
• 发表时间: 2000-02
• 期刊: Endocrine reviews
• 影响因子: 20.3
• 作者: R. Sapolsky;L. Romero;A. Munck

Glucocorticoid receptors and actions.

糖皮质激素受体和作用。

• 发表时间: 1990
• 期刊: The American review of respiratory disease
• 作者: Munck,A;Mendel,DB;Smith,LI;Orti,E
• 通讯作者: Orti,E

Kinetics of glucocorticoid receptor phosphorylation in intact cells. Evidence for hormone-induced hyperphosphorylation after activation and recycling of hyperphosphorylated receptors.

完整细胞中糖皮质激素受体磷酸化的动力学。

• 发表时间: 1993
• 期刊: The Journal of biological chemistry
• 作者: Ortí,E;Hu,LM;Munck,A
• 通讯作者: Munck,A

Isoform composition and stoichiometry of the approximately 90-kDa heat shock protein associated with glucocorticoid receptors.

• DOI: 10.1016/s0021-9258(18)68698-3
• 发表时间: 1988-05
• 期刊: The Journal of biological chemistry
• 作者: D. Mendel;E. Ortí

Glucocorticoid receptors in ATP-depleted cells. Dephosphorylation, loss of hormone binding, HSP90 dissociation, and ATP-dependent cycling.

ATP 耗尽细胞中的糖皮质激素受体。

• 发表时间: 1994
• 期刊: The Journal of biological chemistry
• 作者: Hu,LM;Bodwell,J;Hu,JM;Ortí,E;Munck,A
• 通讯作者: Munck,A