Directed evolution of ancestral bacterial flagellar motors

祖先细菌鞭毛马达的定向进化

• 批准号: DP190100497
• 负责人: A/Prof Matthew Baker
• 金额: $ 28.44万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2022-07-01
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190100497
• 关键词: Directed; evolution; ancestral; bacterial; flagellar

This project aims to produce new knowledge concerning the adaptation of bacterial species to wide environmental changes. The bacterial flagellar motor (BFM) is a motor 40 nanometers in diameter that builds itself into bacterial membranes, rotates five times faster than a Formula One engine, and switches directions in milliseconds. . This project will combine ancestral reconstruction of ancient motor components with protein engineering to understand how the different ion channels that power the BFM in different species are selective for different positive ions. It will inspire and inform future manufacturing in bionanotechnology.

该项目旨在产生关于细菌物种适应广泛环境变化的新知识。细菌鞭毛马达（BFM）是一种直径为40纳米的马达，它将自己构建在细菌膜中，旋转速度比一级方程式发动机快5倍，并在几毫秒内改变方向。该项目将把古代马达部件的祖先重建与蛋白质工程相结合，以了解不同物种中为BFM提供动力的不同离子通道如何选择不同的正离子。它将启发和指导未来的生物纳米技术制造。