CIRCADIAN AND HOMEOSTATIC REGULATION OF REM SLEEP

快速眼动睡眠的昼夜节律和稳态调节

• 批准号: 2889991
• 负责人: SARAH WURTS BLACK
• 金额: $ 2.11万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-03 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2889991
• 关键词: REM sleep; afferent nerve; circadian rhythms; dorsal raphe nucleus; efferent nerve; electroencephalography; electromyography; experimental brain lesion; fos protein; homeostasis; immunocytochemistry; laboratory rat; sleep deprivation; suprachiasmatic nucleus

DESCRIPTION (adapted from applicant's abstract): The proposed experiments will investigate how the circadian system regulates the expression of rapid-eye-movement (REM) sleep. This work offers new basic understanding of REM sleep physiology relevant to the clinical significance of REM sleep disturbances in depression, schizophrenia, narcolepsy, alcoholism, obsessive-compulsive disorder, and Alzheimer's disease. REM sleep has a robust circadian rhythm and appears to be homeostatically regulated in rodents and humans. Although the circadian pacemaker located in the suprachiasmatic nucleus of the hypothalamus (SCN) is thought to promote cortical and behavioral activation and oppose compensatory NREM sleep drive, it is not known how the circadian system interacts with compensatory REM sleep mechanisms. Furthermore, the essential neural connections between the SCN and the REM sleep generator in the brain-stem have not been identified. The experiments in this revised pre-doctoral NRSA proposal will investigate how circadian and homeostatic influences may be integrated to shape REM sleep by: (1) comparing the compensatory REM sleep responses of intact and SCN-lesioned rats to REM sleep deprivation at different circadian times, and (3) examining the convergence of SCN/proximal relay efferent and dorsal raphe afferent projections with FOS and glutamic acid decarboxylase (GAD67) immunohistochemistry in the vlPHA after REM sleep deprivation and compensation.

描述（改编自申请人摘要）：拟定实验 将研究昼夜节律系统如何调节 快速眼动（REM）睡眠 这项工作提供了新的基本认识， 快速眼动睡眠的生理学相关性及临床意义 抑郁症，精神分裂症，嗜睡症，酗酒， 强迫症和老年痴呆症 快速眼动睡眠 强大的昼夜节律，似乎是稳态调节， 啮齿动物和人类。 虽然昼夜节律起搏器位于 下丘脑视交叉上核（SCN）被认为促进 皮质和行为激活并对抗补偿性NREM睡眠驱动， 昼夜节律系统如何与代偿性REM相互作用尚不清楚， 睡眠机制 此外，神经元之间的基本神经连接 SCN和脑干中的REM睡眠发生器尚未确定。 这项修订后的博士前NRSA提案中的实验将进行调查 如何将昼夜节律和自我平衡的影响整合到快速眼动的形成中 睡眠：（1）比较完整的和 SCN损伤大鼠在不同昼夜节律时间的REM睡眠剥夺， (3)检查SCN/近端中继传出和背侧的会聚 FOS和谷氨酸脱羧酶（GAD 67）的中缝传入投射 REM睡眠剥夺后vlPHA的免疫组化， 赔偿