CIRCADIAN AND HOMEOSTATIC REGULATION OF REM SLEEP

描述(改编自申请人的摘要)：建议的实验 将研究昼夜节律系统是如何调节 快速眼动(REM)睡眠。这项工作为我们提供了对 快速眼动睡眠生理学相关的临床意义 抑郁症，精神分裂症，嗜睡症，酗酒， 强迫症和阿尔茨海默氏症。快速眼动睡眠有一个 强健的昼夜节律，似乎是在体内平衡调节的 啮齿动物和人类。尽管昼夜节律起搏器位于 下丘脑视交叉上核(SCN)被认为促进 大脑皮层和行为激活，反对代偿性NREM睡眠驱动， 目前尚不清楚昼夜节律系统如何与代偿性快速眼动相互作用。 睡眠机制。此外，大脑和大脑之间的基本神经联系 SCN和脑干中的REM睡眠生成器尚未确定。 这项修订后的博士前NRSA计划中的实验将调查 昼夜节律和动态平衡的影响如何结合在一起形成REM 睡眠方式：(1)比较正常组和正常组的代偿性快速眼动睡眠反应 SCN损毁大鼠在不同昼夜节律时的REM睡眠剥夺，以及 (3)检查SCN/近端中继神经传出和背侧的会聚 FOS和谷氨酸脱羧酶(GAD67)的中缝传入投射 快速眼动睡眠剥夺后vlPHA的免疫组织化学研究 补偿。

DESCRIPTION (adapted from applicant's abstract): The proposed experiments will investigate how the circadian system regulates the expression of rapid-eye-movement (REM) sleep. This work offers new basic understanding of REM sleep physiology relevant to the clinical significance of REM sleep disturbances in depression, schizophrenia, narcolepsy, alcoholism, obsessive-compulsive disorder, and Alzheimer's disease. REM sleep has a robust circadian rhythm and appears to be homeostatically regulated in rodents and humans. Although the circadian pacemaker located in the suprachiasmatic nucleus of the hypothalamus (SCN) is thought to promote cortical and behavioral activation and oppose compensatory NREM sleep drive, it is not known how the circadian system interacts with compensatory REM sleep mechanisms. Furthermore, the essential neural connections between the SCN and the REM sleep generator in the brain-stem have not been identified. The experiments in this revised pre-doctoral NRSA proposal will investigate how circadian and homeostatic influences may be integrated to shape REM sleep by: (1) comparing the compensatory REM sleep responses of intact and SCN-lesioned rats to REM sleep deprivation at different circadian times, and (3) examining the convergence of SCN/proximal relay efferent and dorsal raphe afferent projections with FOS and glutamic acid decarboxylase (GAD67) immunohistochemistry in the vlPHA after REM sleep deprivation and compensation.