TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
时间分辨磁圆二色性
基本信息
- 批准号:2900657
- 负责人:
- 金额:$ 18.05万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1987
- 资助国家:美国
- 起止时间:1987-07-01 至 2000-03-31
- 项目状态:已结题
- 来源:
- 关键词:biomedical equipment development chemical kinetics circular magnetic dichroism conformation cytochrome b cytochrome c cytochrome oxidase electron transport enzyme mechanism enzyme structure hemoglobin hemoprotein structure magnetic field method development molecular dynamics mutant photochemistry photolysis protein structure function structural biology time resolved data ultraviolet radiation
项目摘要
DESCRIPTION: Development and use of time resolved magnetic circular
dichroism spectroscopy (TRMCD) is proposed. The application of a
magnetic field induces chirality and the observed signal is generally
localized to the moiety who's absorption is being probed. This
technique is used for heme centers. Extension of the technique to the
near UV is to be developed, and preliminary data on the static MCD of
tryptophan is encouraging (will require an extra electromagnet to
compensate for the Faraday rotation of the cell windows). The central
idea is to photolyze a ligand and then study the kinetics. The
resolution of the TRMCD apparatus is about 50-100 ns in the heme center
absorption (partly complicated by signals that result from the protein
diffusional reorientation time, about 100 ns- this complication has been
analyzed and is probably correctable). Studies on cytochrome oxidase,
cytochrome c3, and cytochrome c' are ongoing. A particularly important
finding of the past period was the finding of a transient ligation at the
cytochrome a3 site of cytochrome oxidase, resulting in a model for part
of the gating mechanism coupling energy release by oxygen reduction to
proton pumping (the ligand shuttle model). An unknown ligand in the
protein binds to the protein iron center and this has been best studied
with this technique. Most interesting future studies center on
hemoglobin and the R to T allosteric transition, if the trp residues can
be isolated and measured. Some hope has been demonstrated by static MCD
on trp in solution. Mutants should isolate which trp residues yield
signal. Recent TRCD results, just submitted to Biochemistry, are
exciting. Outstanding studies except there were two problems cited in
last review: the problem of protein orientational relaxation, which is
on the 100 ns time scale, and the issue of productivity.
描述:研制和使用时间分辨磁圆
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DAVID S. KLIGER其他文献
DAVID S. KLIGER的其他文献
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{{ truncateString('DAVID S. KLIGER', 18)}}的其他基金
Fast Kinetic Studies of Protein Folding and Function
蛋白质折叠和功能的快速动力学研究
- 批准号:
7098013 - 财政年份:2004
- 资助金额:
$ 18.05万 - 项目类别:
Fast Kinetic Studies of Protein Folding and Function
蛋白质折叠和功能的快速动力学研究
- 批准号:
6874802 - 财政年份:2004
- 资助金额:
$ 18.05万 - 项目类别:
Fast Kinetic Studies of Protein Folding and Function
蛋白质折叠和功能的快速动力学研究
- 批准号:
7270641 - 财政年份:2004
- 资助金额:
$ 18.05万 - 项目类别:
Fast Kinetic Studies of Protein Folding and Function
蛋白质折叠和功能的快速动力学研究
- 批准号:
6945191 - 财政年份:2004
- 资助金额:
$ 18.05万 - 项目类别:
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