TRANSIENT MAGNETIC CIRCULAR DICHROISM

此应用程序为持续的时间分辨磁性循环寻求资金 生物系统的二色性(TRMCD)研究。前一批款期 证明了用纳秒测量MCD光谱是可能的 时间分辨率。现在有人提议将这项技术应用于 几个具有生物医学重要性的问题。这些问题可分为三个方面： 亚铁血红素配体光解中间体的研究 蛋白质和金属卟啉，电子和质子转移的研究 互变异构化反应和血红素蛋白中的反应及其研究 分子激发态的MCD光谱。其中一些研究是 旨在进一步发展TRMCD技术或开发 解释MCD光谱所需的理论框架。在这些中， 可能会干扰TRMCD测量的伪像将是 进行调查，以确保它们被理解并且可以根据我们的 实验条件。此外，还将研究简单的系统，这将 服从直截了当的理论处理，因此这些 可以对治疗进行优化，以了解MCD光谱特征。多数 然而，这些研究中将使用已经被证明是 可有效研究多种蛋白质系统。这些都是针对 了解配体、电子或质子转移反应的性质 在蛋白质中。这些都是关键的过程，它们负责广泛的 生命所必需的各种过程，从氧气在地球上的运输 血液和肌肉，以生产可用于能源的物种 按细胞分类的来源。

This application seeks funds for continued time-resolved magnetic circular dichroism (TRMCD) studies of biological systems. The previous grant period demonstrated that it is possible to measure MCD spectra with nanosecond time resolution. It is now proposed that this technique be applied to several problems of biomedical importance. These fall into three areas: the study of intermediates which result from ligand photolysis from heme proteins and metalloporphyrins, the study of electron and proton transfer reactions in tautomerization reactions and in heme proteins, and the study of MCD spectra of molecular excited states. A few of these studies are aimed at further development of the TRMCD technique or development of theoretical frameworks needed to interpret MCD spectra. In these, artifacts which could interfere with TRMCD measurements will be investigated to be sure they are understood and can be eliminated under our experimental conditions. Also, simple systems will be studied which will be amenable to straightforward theoretical treatment so that these treatments can be optimized for understanding MCD spectral features. Most of the studies, however, will use the techniques already proven to be effective to study a variety of protein systems. These are aimed at understanding the nature of ligand, electron, or proton transfer reactions in proteins. These are critical processes which are responsible for a wide variety of processes necessary for life from transport of oxygen in the blood and muscles to production of species which can be used for energy sources by cells.