TRANSIENT MAGNETIC CIRCULAR DICHROISM

本申请寻求资金，继续时间分辨磁循环 二向色性（TRMCD）研究生物系统。 上一个赠款期 证明了在纳秒级测量MCD谱是可能的 时间分辨率 现在建议将该技术应用于 几个生物医学上的重要问题。 这些措施分为三个方面： 血红素配体光解中间体研究 蛋白质和金属卟啉，电子和质子转移的研究 在互变异构反应和血红素蛋白中的反应，以及研究 分子激发态的MCD光谱。 其中一些研究是 旨在进一步发展TRMCD技术或发展 理论框架需要解释MCD光谱。 在这些， 可能干扰TRMCD测量的伪影将被 调查，以确保他们被理解，并可以消除根据我们的 实验条件 此外，将研究简单的系统， 可以进行简单的理论处理， 可以优化治疗以理解MCD光谱特征。 最 然而，这些研究中的一部分将使用已经被证明是可行的技术。 有效地研究了各种蛋白质系统。 这些都是针对 理解配体、电子或质子转移反应的性质 在蛋白质中。 这些都是关键的进程， 生命所必需的各种过程，从氧气在大气中的运输， 血液和肌肉生产的物种，可用于能源 细胞的来源。

This application seeks funds for continued time-resolved magnetic circular dichroism (TRMCD) studies of biological systems. The previous grant period demonstrated that it is possible to measure MCD spectra with nanosecond time resolution. It is now proposed that this technique be applied to several problems of biomedical importance. These fall into three areas: the study of intermediates which result from ligand photolysis from heme proteins and metalloporphyrins, the study of electron and proton transfer reactions in tautomerization reactions and in heme proteins, and the study of MCD spectra of molecular excited states. A few of these studies are aimed at further development of the TRMCD technique or development of theoretical frameworks needed to interpret MCD spectra. In these, artifacts which could interfere with TRMCD measurements will be investigated to be sure they are understood and can be eliminated under our experimental conditions. Also, simple systems will be studied which will be amenable to straightforward theoretical treatment so that these treatments can be optimized for understanding MCD spectral features. Most of the studies, however, will use the techniques already proven to be effective to study a variety of protein systems. These are aimed at understanding the nature of ligand, electron, or proton transfer reactions in proteins. These are critical processes which are responsible for a wide variety of processes necessary for life from transport of oxygen in the blood and muscles to production of species which can be used for energy sources by cells.