EAND1H TRANSLOCATION--ENERGY CONVERSION/STRUCTURAL STUDY

EAND1H 易位--能量转换/结构研究

• 批准号: 6018612
• 负责人: J. KENT BLASIE
• 金额: $ 22.32万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-08-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6018612
• 关键词: X ray crystallography; X ray spectrometry; chemical kinetics; cytochrome b; cytochrome c; cytochrome oxidase; electron transport; lipid bilayer membrane; photosynthetic reaction centers; protein structure function

The overall goal of this project is to determine important aspects of the role of intramolecular and intermolecular structure in biological energy conversion. The structural studies proposed here concern the determination of the cylindrically-averaged profile structures if the natural membrane~s~s molecular components within specific bimolecular complexes involved in energy conversion at moderate resolution (about 10 A); the spatial relationships between these components, relative to each other and to the overall profile structure of the membrane; and the spatial relationships among the redox centers associated with these components, relative to each other within these complexes and relative to the overall profile structure of the membrane, with an accuracy of plus/minus1-2A. In addition, analogous structural studies will be extended to the determination of the 3-D structures of three, much less structurally complex, artificial metalloprotein ~maquettes~ designed to mimic key characteristics of such charge- separating redox enzymes. Through a correlation of such structural parameters with the capabilities of these natural and artificial components and/or complexes thereof to exhibit efficient electron transport and the generation of transmembrane electrochemical potentials (and ultimately the synthesis of ATP) in reconstituted, vectorially-oriented single membrane systems, we hope to gain substantial insight into the mechanism of energy coupling in biological membranes.

本项目的总体目标是确定重要方面 分子内和分子间结构的作用， 生物能量转换 建议的结构研究 这里涉及圆柱平均的确定 剖面结构，如果天然膜~s~s分子 特定双分子复合物中的组分， 能量转换在中等分辨率（约10 A）;空间 这些组件之间的关系，相对于彼此， 膜的整体轮廓结构;以及空间 氧化还原中心之间的关系与这些 组分，相对于这些复合物内的彼此， 相对于膜的整体轮廓结构， 精度为± 1 -2A。 此外，类似的结构 研究将扩展到三维结构的确定 三种结构简单的人造金属蛋白 设计成模仿这种炸药的关键特性的模型 分离氧化还原酶。 通过这种相互关联， 结构参数与这些自然和 人工组分和/或其复合物， 电子传递和跨膜产生 电化学电位（并最终合成ATP） 重组，矢量定向单膜系统，我们 希望能深入了解能量的机制 生物膜中的耦合。