EAND1H TRANSLOCATION--ENERGY CONVERSION/STRUCTURAL STUDY

该项目的总体目标是确定重要方面 分子内和分子间结构在分子内和分子间的作用 生物能量转换。建议的结构研究 这里涉及柱面平均数的测定 天然膜的剖面结构~S~S分子 特定双分子络合物中的组分参与了 中等分辨率(约10A)的能量转换；空间 这些组件之间的关系，相对于彼此和 到膜的整体轮廓结构；以及空间 与这些相关的氧化还原中心之间的关系 组件，在这些复合体中彼此相对，并且 相对于膜的整体轮廓结构，具有 正负1-2A的精确度。此外，类似的结构 研究将扩展到确定三维结构 三种结构简单得多的人造金属蛋白 ~模型~设计用于模拟此类炸药的关键特性- 分离氧化还原酶。通过这样的关联 结构参数具有这些天然的和 人工成分和/或其复合体以展示高效 电子传递与跨膜的产生 电化学势(并最终合成三磷酸腺苷) 重组的、面向矢量的单膜系统，我们 希望对能量的机制有实质性的了解 生物膜中的偶联。

The overall goal of this project is to determine important aspects of the role of intramolecular and intermolecular structure in biological energy conversion. The structural studies proposed here concern the determination of the cylindrically-averaged profile structures if the natural membrane~s~s molecular components within specific bimolecular complexes involved in energy conversion at moderate resolution (about 10 A); the spatial relationships between these components, relative to each other and to the overall profile structure of the membrane; and the spatial relationships among the redox centers associated with these components, relative to each other within these complexes and relative to the overall profile structure of the membrane, with an accuracy of plus/minus1-2A. In addition, analogous structural studies will be extended to the determination of the 3-D structures of three, much less structurally complex, artificial metalloprotein ~maquettes~ designed to mimic key characteristics of such charge- separating redox enzymes. Through a correlation of such structural parameters with the capabilities of these natural and artificial components and/or complexes thereof to exhibit efficient electron transport and the generation of transmembrane electrochemical potentials (and ultimately the synthesis of ATP) in reconstituted, vectorially-oriented single membrane systems, we hope to gain substantial insight into the mechanism of energy coupling in biological membranes.