POLARIZED XAFS ON VECTORIALLY ORIENTED SINGLE MONOLAYERS OF CYTOCHROME C

细胞色素 C 向量定向单分子层上的偏振 XAFS

• 批准号: 6205767
• 负责人: J. KENT BLASIE
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205767
• 关键词: biological products; biomedical resource; enzymes; spectrometry

We have been continuing our investigations of the low spin iron center in nitrile hydratase, in collaboration with Mark Nelson and John Cummings of DuPont, who have provided us with frozen protein samples. EXAFS has identified two or three sulfur ligands to the iron center, with the remaining ligands being oxygen or nitrogen scatterers. Some minor changes are seen with binding of inhibitors (to the enzymenot necessarily to the iron center) such as phenylhydrazine or isobutyronitrile. We are currently in the process of modelling the EXAFS to give bond length distributions to see if this may give insight into the changes which are occuring on the binding of these inhibitors. SCIENTICIC SUBPROJECT 61

我们一直在继续我们对低自旋铁的研究 与Mark Nelson和Mark Nelson合作 杜邦公司的约翰·卡明斯为我们提供了冷冻蛋白质 样本。EXAFS已经确定了铁的两到三个硫配体 中心，其余的配体是氧或氮 散布者。与抑制剂的结合可以看到一些微小的变化。 (到酶不一定到铁中心)例如 苯肼或异丁腈。我们目前正在进行这项工作 对EXAFS进行建模，给出键长分布，看看是否 这可能会让我们深入了解正在发生的变化 这些抑制物的结合。SCIENTICIC子项目61