ENERGY CONVERSION

这项研究项目的目的是调查 偶联反应中的分子内和分子间结构 生物中电子和质子转运对三磷酸腺苷合成的影响 膜。通过对整体形状的确定 中等分辨率的相关膜分子组成 以及它们的位置(包括它们的氧化还原位置 中心)相对于彼此以及相对于整个膜 结构，预计将获得实质性的洞察力 关于电子和质子的可能机制 移位和能量耦合。非标的技术 共振和共振X射线和中子衍射法 (扩展X射线吸收精细结构)光谱，光学 线性二色性、三维电子显微镜图像 重建和FRAP(之后的荧光恢复 光漂白)将被用于研究相关的膜 蛋白质包括光合作用反应中心、细胞色素 氧化酶、细胞色素c和细胞色素b/c1及各种 其络合物主要以单一、定向的形式存在 单层膜系统和在某些情况下定向 膜多层系统。反应中心--细胞色素 B/c1-细胞色素c络合物表现出高效的光驱动 一系列循环的电子转移反应。

The purpose of this research project is to investigate the role of intramolecular and intermolecular structure in the coupling of electron and proton translocation to ATP synthesis in biological membranes. Through a determination of the overall shapes of relevant membrane molecular components at moderate resolution and of their positions (including the positions of their redox centers) relative to each other and to the overall membrane structure, it is expected that substantial insight will be gained concerning the possible mechanisms of electron and proton translocation and of energy coupling. The techniques of non- resonance and resonance X-ray and neutron diffraction, EXAFS (Extended X-ray Absorption Fine Structure) spectroscopy, optical linear dichroism, three-dimensional electron microscopic image reconstruction, and FRAP (Fluorescence Recovery After Photobleaching) will be utilized to study the relevant membrane proteins including photosynthetic reaction centers, cytochrome oxidase, cytochrome c and cytochrome b/c1 and various complexes thereof primarily in the form of single, oriented monolayer membrane systems and in some instances oriented membrane multilayer systems. The reaction center-cytochrome b/c1-cytochrome c complexes exhibit an efficient light-driven cyclic series of electron transfer reactions.