REGULATION OF FILAMENTOUS GROWTH IN YEAST
REGULATION OF FILAMENTOUS GROWTH IN YEAST
批准号:
2857263
负责人:
Haoping Liu
金额:
$20.26万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-01-01 至 2001-12-31
中文摘要
这项建议的目的是了解提供
对收敛的MAP激酶通路的特异性。信息素反应
酿酒酵母中负责传播的途径
在单倍体接合过程中,胞外交配信号进入细胞核。
它包括信息素受体,一种异三聚体G蛋白,一种高度
保守的PAK/Mekk/MEK/MAPK激酶级联(Ste20p、Ste11p、Ste7p和
Fus3p/Kss1p)和转录因子Ste12p。中的元素
还需要路径,包括Ste20p、Ste11p、Ste7p和Ste12p
用于二倍体丝状生长(细长细胞链的发育
氮素饥饿时)和单倍体入侵生长。因此,一个激酶
在单倍体和单倍体中,不同的发育途径使用级联
二倍体和途径的输出涉及相同的转录
因数,STE12P。这项拟议的研究使用了分子和基因
确定Ste12p调节器和目标的方法,并了解
STE_(12)P在丝状细胞生长中的调控作用及其比较
在单倍体交配中。第一个具体目标是识别和描述
Ste12p调控丝状二倍体细胞中的基因。基因
丝状细胞生长过程中Ste12p的转录调控
二倍体将用酵母LacZ融合文库克隆。他们的
表达模式、蛋白质定位和突变表型将是
分析过了。一些克隆基因的启动子将被定位以定义
STE 12P诱导的上游激活序列
抄写。第二个目的是了解STE12P的机制
对丝状菌生长的监管,并确定其监管机构。我们建议
研究丝状化条件下Ste12p的磷酸化。遗传
筛选的目的是识别二倍体中的Ste12p激酶,因为
信息素反应途径的MAP激酶Fus3p/Kss1不是必需的
用于丝状生长。我们建议增加监管机构的职能
与Stel2p一起提供了二倍体的特异性调控。两者都有
基因和生化实验将被用来鉴定这些
监管者。保守的MAP激酶对Ste12p调控的研究
路径解决了一个有趣的问题,涉及到对
在这些途径中具有特异性。这项研究将加强我们的
对MAP激酶通路复杂调控机制的认识
哺乳动物细胞。基因二态调控的研究进展
易驯化的酿酒酵母也将加深我们对真菌的了解
二形性在白色念珠菌的发病机制中很重要,
人类常见的真菌病原体。
英文摘要
The aim of this proposal is to understand mechanisms that provide the
specificity to converging MAP kinase pathways. The pheromone response
pathway in Saccharomyces cerevisiae is responsible for transmitting
extracellular mating signals into the nucleus during haploid conjugation.
It includes pheromone receptors, a hetero-trimeric G protein, a highly
conserved PAK/MEKK/MEK/MAPK kinase cascade (Ste20p, Ste11p, Ste7p, and
Fus3p/Kss1p), and a transcription factor, Ste12p. Elements of the
pathway, including Ste20p, Ste11p, Ste7p, and Ste12p, are also required
for diploid filamentous growth (development of chains of elongated cells
upon nitrogen starvation) and haploid invasive growth. Thus, one kinase
cascade is used by different developmental pathways in haploids and
diploids and the outputs of the pathways involve the same transcription
factor, Ste12p. The proposed research uses molecular and genetic
approaches to identify Ste12p regulators and targets, and to understand
Ste 12p regulation in filamentous growth in comparison to its regulation
in haploid mating. The first specific aim is to identify and characterize
Ste12p regulated genes in filamentous diploid cells. Genes
transcriptionally regulated by Ste12p during filamentous growth in
diploids will be cloned with a yeast lacZ fusion library. Their
expression pattern, protein localization and mutant phenotypes will be
analyzed. Promoters of some of the cloned genes will be mapped to define
the upstream activation sequences responsible for Ste 12p induced
transcription. The second aim is to understand the mechanism of Ste12p
regulation in filamentous growth and identify its regulators. We propose
to study Ste12p phosphorylation under filamentation conditions. Genetic
screens are designed to identify a Ste12p kinase in diploids because the
MAP kinases Fus3p/Kss1 of the pheromone response pathway are not required
for filamentous growth. We propose additional regulators functioning
together with Stel2p provide the diploid specific regulation. Both
genetic and biochemical experiments will be utilized to identify these
regulators. Our research on Ste12p regulation by the conserved MAP kinase
pathway addresses an interesting question concerning the regulation of
specificity among these pathways. This study will enhance our
understanding of the complicated regulation by MAP kinase pathways in
mammalian cells. Investigation of dimorphic regulation in genetically
tractable S. cerevisiae will also further our knowledge of fungal
dimorphism, which is important in the pathogenesis of Candida albicans,
a common fungal pathogen of humans.
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会议论文
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批准号:10390180
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项目类别:
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资助金额:$1.72万
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财政年份:2021
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Signal sensing, hyphal development and pathogenesis of Candida albicans
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批准号:9263215
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财政年份:2017
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Signal sensing, hyphal development and pathognesis of Candida albicans
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批准号:10596976
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项目类别:
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资助金额:$48.03万
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财政年份:2017
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批准号:10362712
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项目类别:
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资助金额:$48.03万
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财政年份:2017
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负责人:Haoping Liu
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依托单位:
Signal sensing, hyphal development and pathogenesis of Candida albicans
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批准号:10810526
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项目类别:
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资助金额:$1.11万
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财政年份:2017
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负责人:Haoping Liu
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依托单位:
Dissecting triazole resistance and transcriptional regulation of ergosterol homeo
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批准号:8447403
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项目类别:
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资助金额:$59.61万
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财政年份:2012
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负责人:Haoping Liu
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依托单位:
Dissecting triazole resistance and transcriptional regulation of ergosterol homeo
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批准号:8282580
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项目类别:
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资助金额:$63.21万
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财政年份:2012
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负责人:Haoping Liu
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依托单位:
Dissecting triazole resistance and transcriptional regulation of ergosterol homeo
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批准号:8636992
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项目类别:
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资助金额:$63.62万
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财政年份:2012
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负责人:Haoping Liu
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依托单位:
Dissecting triazole resistance and transcriptional regulation of ergosterol homeo
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批准号:8831447
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项目类别:
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资助金额:$63.68万
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财政年份:2012
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负责人:Haoping Liu
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依托单位:
Regulation of Filamentous Growth in Yeast
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批准号:7989655
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项目类别:
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资助金额:$2.13万
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财政年份:2009
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负责人:Haoping Liu
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依托单位:
IDENTIFICATION OF PROTEINS THAT INTERACT WITH STE12P
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批准号:6979530
-
项目类别:
-
资助金额:$0.36万
-
财政年份:2004
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负责人:Haoping Liu
-
依托单位:
CDC24-ASSOCIATED PROTEINS IN YEAST AND CANDIDA
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批准号:6979539
-
项目类别:
-
资助金额:$0.36万
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财政年份:2004
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负责人:Haoping Liu
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依托单位:
IDENTIFY EFG1-ASSOCIATED PROTEINS IN CANDIDA ALBICANS
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批准号:6979538
-
项目类别:
-
资助金额:$0.36万
-
财政年份:2004
-
负责人:Haoping Liu
-
依托单位:
REGULATION OF FILAMENTOUS GROWTH IN YEAST
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批准号:6479011
-
项目类别:
-
资助金额:$27.58万
-
财政年份:1997
-
负责人:Haoping Liu
-
依托单位:
Regulation of Filamentous Growth in Yeast
-
批准号:8523904
-
项目类别:
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资助金额:$33.23万
-
财政年份:1997
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负责人:Haoping Liu
-
依托单位:
REGULATION OF FILAMENTOUS GROWTH IN YEAST
-
批准号:6857053
-
项目类别:
-
资助金额:$27.5万
-
财政年份:1997
-
负责人:Haoping Liu
-
依托单位:
Regulation of Filamentous Growth in Yeast
-
批准号:7098353
-
项目类别:
-
资助金额:$30.86万
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财政年份:1997
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负责人:Haoping Liu
-
依托单位:
Regulation of Filamentous Growth in Yeast
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批准号:8324219
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项目类别:
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资助金额:$34.44万
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财政年份:1997
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负责人:Haoping Liu
-
依托单位:
REGULATION OF FILAMENTOUS GROWTH IN YEAST
-
批准号:6625791
-
项目类别:
-
资助金额:$27.56万
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财政年份:1997
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负责人:Haoping Liu
-
依托单位:
Regulation of Filamentous Growth in Yeast
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批准号:7188565
-
项目类别:
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资助金额:$29.92万
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财政年份:1997
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负责人:Haoping Liu
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依托单位:
海外基金