A novel biocatalyst platform for biobased-synthesis of 1,3-propanediol

生物制造已成为化学催化的一种有前途的替代方法，用于生物燃料、药物和精细化学品的绿色、可再生、复杂的合成。与体内发酵方法相比，无细胞化学生物合成提供了额外的优势，通过使单独生产的酶能够即插即用组装成最佳级联、通用反应条件和直接进入反应环境。FabricNano开发了合成生物学，DNA-的纳米反应器平台技术，有可能改变英国的化学合成工业。他们的方法大大提高了反应动力学，多酶级联动力学，快速生产产品，并有助于化学生产的绿色化和可持续性。FabricNano已经确定了一种多酶级联，将废甘油转化为1，3-丙二醇（PDO）。生物乙醇/生物柴油生产产生废甘油，价值为20 p/kg; PDO的价值为9英镑/公斤，由于它在许多应用中的效用（塑料，化妆品，个人护理，清洁产品）对PDO的需求很大，但只有四家领先的制造商有年产量。142.5千吨/年。PDO化学加工技术是能源密集型的，消耗金属催化剂，并产生危险的工作条件。最先进的方法通过发酵将糖和多元醇转化为PDO。然而，发酵依赖于漫长的酵母培养，并消耗大量的能源和淡水。由于新冠肺炎危机限制了英国生物柴油/生物乙醇生产商的市场（休闲旅游和货运服务减少降低了需求），FabricNano发现了一个机会：1.通过为低价值废物流创造附加值，帮助生物乙醇/生物柴油生产商在新冠肺炎危机期间保持盈利/可行的运营商。为PDO创建国内供应链（目前在英国没有生产商）。该项目将有助于证明FabricNano生物催化剂在工业规模上的可行性。它还将证明FabricNano技术用于耦合级联酶反应以将低价值可再生原料转化为高价值产品的实用性，这代表了可再生绿色化学的关键。

Biomanufacturing has emerged as a promising alternative to chemocatalysis for green, renewable, complex synthesis of biofuels, medicines, and fine chemicals. Cell-free chemical biosynthesis offers additional advantages over in vivo fermentation methods, by enabling plug-and-play assembly of separately produced enzymes into an optimal cascade, versatile reaction conditions, and direct access to the reaction environment.FabricNano have developed a synthetic biology, DNA-based nanoreactor platform technology with the potential to transform the UK's chemical synthesis industry.Their approach vastly improves reaction kinetics of multi-enzyme cascade kinetics to yield product quickly, and can contribute to the greening and sustainability of chemical production.FabricNano have identified a multi-enzyme cascade that converts waste glycerine into 1,3-Propanediol (PDO).Bioethanol/biodiesel production creates waste glycerine, valued at 20p/kg; PDO is valued at £9/kg, due to it utility across many applications (plastics, cosmetics, personal care, cleaning products)There is significant demand for PDO, but just four leading manufacturers have an annual output c. 142.5 kilotons/year. PDO chemical processing techniques are energy intensive, consume metal catalysts, and create dangerous working conditions. State-of-the-art approaches convert sugars and polyols to PDO via fermentation. However, fermentation relies on lengthy yeast culture, and consumes significant energy and fresh water.Because the Covid crisis is restricting the market for the UK's biodiesel/bioethanol producers (less leisure travel and freight services has lowered demand), FabricNano have identified an opportunity to:1. Help bioethanol/biodiesel producers to remain profitable/viable operators during the Covid crisis by creating added-value for a low-value waste stream.2. Create a domestic supply chain for PDO (currently no producers in the UK).The project will help to demonstrate the viability of FabricNano's biocatalyst at an industrial scale. It will also prove utility of FabricNano technology for coupling cascading enzyme reactions for the conversion of low value renewable feedstocks into high value products, which represents a keystone of renewable green chemistry.