A novel biocatalyst platform for biobased-synthesis of 1,3-propanediol

用于生物基合成1,3-丙二醇的新型生物催化剂平台

• 批准号: 96730
• 金额: $ 52.19万
• 依托单位: FABRICNANO LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=96730
• 关键词: novel; biocatalyst; platform; biobased; synthesis

Biomanufacturing has emerged as a promising alternative to chemocatalysis for green, renewable, complex synthesis of biofuels, medicines, and fine chemicals. Cell-free chemical biosynthesis offers additional advantages over in vivo fermentation methods, by enabling plug-and-play assembly of separately produced enzymes into an optimal cascade, versatile reaction conditions, and direct access to the reaction environment.FabricNano have developed a synthetic biology, DNA-based nanoreactor platform technology with the potential to transform the UK's chemical synthesis industry.Their approach vastly improves reaction kinetics of multi-enzyme cascade kinetics to yield product quickly, and can contribute to the greening and sustainability of chemical production.FabricNano have identified a multi-enzyme cascade that converts waste glycerine into 1,3-Propanediol (PDO).Bioethanol/biodiesel production creates waste glycerine, valued at 20p/kg; PDO is valued at £9/kg, due to it utility across many applications (plastics, cosmetics, personal care, cleaning products)There is significant demand for PDO, but just four leading manufacturers have an annual output c. 142.5 kilotons/year. PDO chemical processing techniques are energy intensive, consume metal catalysts, and create dangerous working conditions. State-of-the-art approaches convert sugars and polyols to PDO via fermentation. However, fermentation relies on lengthy yeast culture, and consumes significant energy and fresh water.Because the Covid crisis is restricting the market for the UK's biodiesel/bioethanol producers (less leisure travel and freight services has lowered demand), FabricNano have identified an opportunity to:1. Help bioethanol/biodiesel producers to remain profitable/viable operators during the Covid crisis by creating added-value for a low-value waste stream.2. Create a domestic supply chain for PDO (currently no producers in the UK).The project will help to demonstrate the viability of FabricNano's biocatalyst at an industrial scale. It will also prove utility of FabricNano technology for coupling cascading enzyme reactions for the conversion of low value renewable feedstocks into high value products, which represents a keystone of renewable green chemistry.

生物制造已成为化学催化的一种有前途的替代方案，用于生物燃料、药物和精细化学品的绿色、可再生、复杂合成。无细胞化学生物合成比体内发酵方法具有更多优势，可以将单独生产的酶即插即用组装成最佳级联、多功能反应条件以及直接进入反应环境。FabricNano 开发了一种合成生物学、基于 DNA 的纳米反应器平台技术，有可能改变英国的化学合成行业。他们的方法极大地改善了反应 多酶级联动力学可快速产生产物，并有助于化工生产的绿色化和可持续性。FabricNano已经确定了一种多酶级联，可将废甘油转化为1,3-丙二醇（PDO）。生物乙醇/生物柴油生产会产生废甘油，价值为20p/kg； PDO 的价值为 9 英镑/公斤，因为它适用于许多应用（塑料、化妆品、个人护理、清洁产品）。对 PDO 的需求很大，但只有四家领先的制造商的年产量约为 100 英镑。 142.5 千吨/年。 PDO 化学加工技术属于能源密集型，会消耗金属催化剂，并会产生危险的工作条件。最先进的方法通过发酵将糖和多元醇转化为 PDO。然而，发酵依赖于漫长的酵母培养，并消耗大量能源和淡水。由于新冠危机限制了英国生物柴油/生物乙醇生产商的市场（休闲旅行和货运服务减少导致需求下降），FabricNano 发现了一个机会：1。通过为低价值废物流创造附加值，帮助生物乙醇/生物柴油生产商在新冠危机期间保持盈利/生存能力。2。创建PDO的国内供应链（目前英国没有生产商）。该项目将有助于展示FabricNano生物催化剂在工业规模上的可行性。它还将证明 FabricNano 技术在耦合级联酶反应方面的实用性，可将低价值的可再生原料转化为高价值的产品，这代表了可再生绿色化学的基石。