UNDERSTANDING COMPLEX GENE EDITING SYSTEMS IN PROTISTS

描述：这是一项研究人类免疫缺陷的机制和演变的建议。 黏菌线粒体基因的广泛RNA编辑和耐人寻味 体细胞大核发生在纤毛虫体内的DNA重排 由生殖系微核形成。数学模型将会 描述其中的RNA编辑和DNA重排过程 还将对生物进行调查。 本研究项目由三部分组成。第一部分是关于 随着线粒体RNA编辑进化的重构 粘菌中的基因和纤毛虫中的DNA重排系统 系统发育学方法。为此，私家侦探打算建立一个 不同种类粘菌和粘菌的可靠系统发育树 纤毛虫的核糖体RNA序列，然后推断四种的进化 不同类型的RNA编辑和不同类型的DNA重排 使用简约方法的粘菌和纤毛虫的历史。大卫博士 普雷斯科特已经同意在这个项目上进行合作。 研究的第二部分是一项实验研究，以了解 丘疹螺旋体DNA重排的分子机制。现在是时候了 已知一些生殖系基因由介入性的非编码DNA组成 序列(内部消除的序列；Less)和大核 序列(MDS)和大核形成过程中的序列 IE被取消，MDS以奇怪的顺序组合形成 功能基因。然而，目前尚不清楚MDS是如何按顺序重新排列的 这与胚系基因中MDS的原始顺序不同。这个 P.I.计划通过一系列研究DNA重排的机制 涉及DNA转化、遗传分析和聚合酶链式反应的功能实验 分析重排的时间进程以测试特定的方面 不同的重排模式。这部分工作将在 汉斯·利普斯博士在德国的实验室。 研究项目的第三部分是关于数学的 RNA编辑和DNA重排的建模。据称，这些DNA 重排过程类似于有向哈密顿路径问题 数学上，重新排列在数学上是非常有效的。这个 P.I.现在想要开发一种更好的数学理论来描述这一点 进程。这部分工作将与L·L博士合作完成。 Kari(西安大略大学)和G.Rozenberg博士(莱顿 大学)。

DESCRIPTION: This is a proposal to study the mechanism and evolution of the extensive RNA editing in mitochondrial genes in slime molds and the intriguing DNA rearrangement that occurs in ciliates when the somatic macronucleus is formed from the germline micronucleus. Mathematical models that would describe the RNA editing and the DNA rearrangement processes in these organisms will also be investigated. This research project consists of three parts. The first part is concerned with the reconstruction of the evolution of the RNA editing of mitochondrial genes in slime molds and the DNA rearrangement systems in ciliates using a phylogenetic approach. For this purpose, the P.I. intends to construct a reliable phylogenetic tree for different groups of species of slime molds and ciliates using ribosomal RNA sequences and then infer the evolution of four different types of RNA editing and various types of DNA rearrangements in the history of slime molds and ciliates using the parsimony method. Dr. David Prescott has agreed to collaborate in this project. The second part of the research is an experimental study to understand the molecular mechanisms of DNA rearrangement in Stylonychia lemnae. It is now known that some germline genes are composed of intervening non-coding DNA sequences (internally eliminated sequences; IESs) and macronuclear destined sequences (MDSs) and that in the process of formation of the macronucleus IES's are eliminated and MDSs are combined in a strange order to form functional genes. However, it is unknown how MDSs are rearranged in the order which is different from the original order of MDSs in the germline gene. The P.I. plans to study the mechanisms of DNA rearrangement through a series of functional experiments involving DNA transformation, genetic analysis, and PCR analysis of the time-course of rearrangement to test specific aspects of different models of rearrangements. This part of the work will be done in the laboratory of Dr. Hans Lipps in Germany. The third part of the research project is concerned with the mathematical modeling of RNA editing and DNA rearrangement. It is stated that the DNA rearrangement process is similar to a directed Hamiltonian path problem in mathematics and that the rearrangement is mathematically very efficient. The P.I. now wants to develop a better mathematical theory for describing this process. This part of the work will be done in collaboration with Dr. L. Kari (University of Western Ontario) and Dr. G. Rozenberg (Leiden University).