VIDEO IMAGING OF SKIN PATHOLOGY USING POLARIZED LIGHT

描述(逐字摘自申请者摘要)：假设 病理组织。首席调查员是从以前的NIH发展而来的 资助(R29HL45045)基于散射偏振光的摄像机系统 这一点已经在试点临床试验中得到证明，以改善医生的 皮肤的表皮和乳头状真皮的可视化。方法 消除皮肤中黑色素沉着的影响，并在 没有黑色素着色的基于偏振光子的图像 从浅表皮肤层内散布，在那里产生了许多病理。 因此，医生查看的图像优化了一个人直接 不受干扰地识别浅层皮肤的细胞结构 由于黑色素沉着而被吸收。例如，黑色素痣 变得清晰，表皮/表皮下间隙中的微妙特征变得 看得见。这些图像令人信服，但也有适当的诠释和未来 摄像机系统的优化需要基本了解如何 偏振光从组织的超微结构散射而来。这项提案已经 两个目标。目标1将阐述对比度和深度的基本基础 对偏振摄像机图像的审问。AIM 2将调查如何 偏振光散射的波长和角度相关性可以指定 一种独特的指纹，它表征了 组织超微结构中的光散射结构。没有人或活体 动物实验也在计划之中。由聚苯乙烯组成的体模组织 从屠宰场获得的微球和动物组织样本将 学习。计算机模拟将是对实验的补充。

DESCRIPTION (Verbatim from the Applicant's Abstract): The hypothesis of this pathologic tissue. The principal investigator has developed from previous NIH funding (R29HL45045) a video camera system based on scattered polarized light which has been shown in pilot clinical trials to improve the doctor's visualization of the epidermis and papillary dermis of the skin. The method eliminates the influence of melanin pigmentation in the skin and creates on image that is devoid of melanin pigmentation and based on polarized photons scattered from within the superficial skin layer where much pathology arises. Hence, the doctor views an image which optimizes one's ability to directly discern the cellular structure of the superficial skin without interference from absorption due to melanin pigmentation. For example, dark pigmented nevi become clarified and subtle features in the epidermal/subepidermal space become visible. The images are compelling, but proper interpretation and future optimization of the camera system require a basic understanding of how polarized light scatters from the ultrastructure of tissues. The proposal has two aims. Aim 1 would address the underlying basis for contrast and depth of interrogation of the polarized video camera images. Aim 2 would investigate how the wavelength and angular-dependence of polarized light scattering can specify a unique fingerprint that characterizes the size and number density of light-scattering structures in the tissue ultrastructure. No human or in vivo animal studies are planned. Phantom tissues composed of polystyrene microspheres and animal tissue samples obtained from the abattoir will be studied. Computer simulations will complement experiments.