VIDEO IMAGING OF SKIN PATHOLOGY USING POLARIZED LIGHT

描述（来自申请人摘要的逐字描述）： 病理组织首席研究员从以前的NIH 资助（R29 HL 45045）基于散射偏振光的摄像系统 这已经在试点临床试验中证明， 皮肤的表皮和乳头状真皮的可视化。述的方法 消除皮肤黑色素沉着的影响， 没有黑色素沉着且基于偏振光子成像 从产生许多病理的浅表皮肤层内分散。 因此，医生查看图像，该图像优化了直接 辨别皮肤表层的细胞结构而不受干扰 由于黑色素沉着而被吸收。例如，暗色素痣 变得清晰，表皮/表皮下空间的细微特征变得 可见这些图像是引人注目的，但适当的解释和未来 摄像机系统的优化需要基本了解如何 偏振光从组织的超微结构散射。该提案 两个目标。目标1将涉及对比和深度的基本基础， 偏振摄像机图像的询问。目标2将研究如何 偏振光散射的波长和角度依赖性可以指定 一个独特的指纹，其特征在于 组织超微结构中的光散射结构。无人体或体内 计划进行动物研究。由聚苯乙烯组成的体模组织 从屠宰场获得的微球和动物组织样品将 研究了计算机模拟将补充实验。

DESCRIPTION (Verbatim from the Applicant's Abstract): The hypothesis of this pathologic tissue. The principal investigator has developed from previous NIH funding (R29HL45045) a video camera system based on scattered polarized light which has been shown in pilot clinical trials to improve the doctor's visualization of the epidermis and papillary dermis of the skin. The method eliminates the influence of melanin pigmentation in the skin and creates on image that is devoid of melanin pigmentation and based on polarized photons scattered from within the superficial skin layer where much pathology arises. Hence, the doctor views an image which optimizes one's ability to directly discern the cellular structure of the superficial skin without interference from absorption due to melanin pigmentation. For example, dark pigmented nevi become clarified and subtle features in the epidermal/subepidermal space become visible. The images are compelling, but proper interpretation and future optimization of the camera system require a basic understanding of how polarized light scatters from the ultrastructure of tissues. The proposal has two aims. Aim 1 would address the underlying basis for contrast and depth of interrogation of the polarized video camera images. Aim 2 would investigate how the wavelength and angular-dependence of polarized light scattering can specify a unique fingerprint that characterizes the size and number density of light-scattering structures in the tissue ultrastructure. No human or in vivo animal studies are planned. Phantom tissues composed of polystyrene microspheres and animal tissue samples obtained from the abattoir will be studied. Computer simulations will complement experiments.