ROLE OF TUMOR NECROSIS FACTOR-ALPHA IN DEVELOPMENT

描述（改编自研究者摘要）：长期目标 这项研究的目的是确定TNF基因家族在 生殖 新的实验方法已经开发出来， 评估滋养层细胞TNFa的功能的目的，包括 使用携带TNFa/LTa和TNF缺失的敲除（KO）小鼠 受体（TNF-R）基因，并产生小鼠滋养层细胞系 和正常小鼠一样。 初步实验表明：1） TNF信号系统对胎盘的正常发育至关重要 2）TNF/TNF-R信号转导参与性别特异性途径 影响正常发育，3）滋养层细胞系， 小鼠胎盘表达TNF α基因，4）在某些条件下， 滋养层细胞系的生长受到外源性TNF α的抑制。 额外 研究表明，TNF的另外两个生物有效成员 FasL和Lt-beta在子宫和胎盘中表达， 怀孕的小鼠，初步数据强烈表明，FasL具有 在限制母胎迁移中的重要作用 造血细胞

DESCRIPTION (Adapted from the Investigator's Abstract): The long range goal of this research is to define the role(s) of the TNF gene family in reproduction. New experimental approaches have been developed for the purpose of evaluating the functions of trophoblast cell TNFa that include use of knockout (KO) mice carrying deletions of the TNFa/LTa and TNF receptor (TNF-R) genes, and generation of mouse trophoblastic cell lines from these as well as normal mice. Preliminary experiments show that: 1) the TNF signaling system is critical to normal development of placental compartments, 2) TNF/TNF-R signaling is involved in gender-specific pathways that affect normal development, 3) trophoblastic cell lines derived from mouse placentas express the TNFa gene and, 4) under some conditions, trophoblastic cell lines are growth-inhibited by exogenous TNFa. Additional studies demonstrate that two other biologically-potent members of the TNF superfamily, FasL and Lt-beta, are expressed in the uteri and placentae of pregnant mice, and preliminary data strongly suggest that FasL has an important role in restricting maternal-fetal migration of activated hematopoietic cells.