MURINE COMPLEMENT RECEPTOR CR2

这项拟议的研究将描述小鼠补体受体Cr2。 该基因产生两种相关的蛋白质，它们的主要功能是 被描述为补体调理片段的受体。在一次 病毒或细菌感染时，补体途径与两者都有关 溶解入侵的病原体，并在表面覆盖蛋白质 亚基主要来源于C3产物。这些结合蛋白 然后片段可以被各种不同的受体链识别 使病原体内化并有望中和。这条路， 然而，可以被艾滋病毒等病原体利用，这些病原体基本上可以 劫持补体途径以进入缺乏补体的细胞 合适的受体。 我们计划将重点放在基因和基因产品的两个相关方面。 首先是描述那些促进基因表达的基因控制序列 小鼠B细胞和滤泡树突状细胞中Cr2的表达及意义 抑制其在几乎所有其他细胞类型中的表达。这个问题 将通过创造各种基因结构和 使用体外和体内技术对它们进行分析。第二 研究的阶段是确定Cr2蛋白在 获得对入侵病原体的免疫反应。这些实验 将重点放在Cr2链促进抗原摄取的作用上 通过B细胞和蛋白对滤泡树突状细胞的作用。 我们从这些研究中获得的生物信息应该是 易于理解人CR2作为小鼠的功能(S) 蛋白。

The proposed research will describe the murine complement receptor Cr2. This gene produces two related proteins whose primary functions have been described as receptors for opsonized fragments of complement. During a viral or bacterial infection, the complement pathway is engaged to both lyse the invading pathogen as well as to coat the surface with protein subunits derived primarily from the C3 product. These bound protein fragments can then be recognized by a variety of different receptor chains to internalize and hopefully neutralize the pathogen. This pathway, however, can be exploited by pathogens such as HIV which can essentially hijack the complement pathway to gain entry into cells for which they lack the appropriate receptor. We plan to focus upon two related aspects of the gene and gene products. First is to describe those genetic control sequences which promote the expression of Cr2 in murine B cells and follicular dendritic cells and inhibit its expression in virtually all other cell types. This question will be approached by creating a variety of genetic constructs and analyzing them using both in vitro and in vivo techniques. The second phase of the research is to determine the role of the Cr2 proteins in the acquisition of an immune response to invading pathogens. These experiments will focus upon the role of the Cr2 chains to promote the uptake of antigen by B cells and the role of the protein on the follicular dendritic cell. The biological information we obtain from these studies should just as amenable to understanding the function(s) of human CR2 as the murine protein.