BIOCHEMISTRY OF TYPE XVI COLLAGEN

XVI 型胶原蛋白的生物化学

• 批准号: 2900796
• 负责人: MON-LI H. CHU
• 金额: $ 24.23万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2900796
• 关键词: animal genetic material tag; collagen; developmental genetics; gene induction /repression; gene mutation; genetic promoter element; laboratory mouse; nucleic acid sequence; oligonucleotides; protein structure function; recombinant proteins; reproductive system; tissue /cell culture

The long term goal of this application is to understand the biological function of type XVI collagen. Collagens, which are major structural macromolecules of connective tissue, consist of at least 19 distinct types. Type XVI collagen is a recently discovered member of the collagen family. It possesses several structural features characteristic of the fibril associated collagen subgroup, whose members include type IS, XII, XIV, and XIX collagens. However, it is unique among collagens in that its long collagenous region is interrupted by multiple noncollagenous domains enriched in cysteines. Type XVI collagen mRNA is expressed at low levels in most tissues but at much higher levels in overy, testis and prosate tissue, suggesting that type XVI collagen plays critical roles in male and female reproductive organs. In this renewal application, experiemtns are proposed to continue investigating the function of type XVI collagen. The expression of type XVI collagenmRNa and protein in male and female reproductive organs will be examined in detail. Transgenic mice with the type XVI collagen gene inactivated or mutated will be prepared to study the in vivo function of type XVI collagen. Recombinant type XVI collagen chains will be prepared to study their binding interactions with other extracellular matrix proteins. The transcription regulation type XVI collagen gene will be studied by characterization of the promoter region of the gene.

该应用程序的长期目标是了解生物 XVI 型胶原蛋白的功能。 胶原蛋白是主要的结构 结缔组织大分子，由至少 19 种不同的 类型。 XVI 型胶原蛋白是最近发现的胶原蛋白成员 胶原蛋白家族。 它具有多种结构特点 原纤维相关胶原亚群的成员，其成员包括类型 IS、XII、XIV 和 XIX 胶原蛋白。 然而，它却是独一无二的 胶原蛋白，因为它的长胶原区域被多个 富含半胱氨酸的非胶原结构域。 XVI型胶原蛋白 mRNA 在大多数组织中表达水平较低，但表达水平却高得多 睾丸、睾丸和前列腺组织中的水平，表明 XVI 型 胶原蛋白在男性和女性生殖器官中起着至关重要的作用。 在此续展申请中，建议继续进行实验 研究 XVI 型胶原蛋白的功能。 的表达式为 男性和女性生殖中的 XVI 型胶原 mRNA 和蛋白质 器官将被详细检查。 XVI型转基因小鼠 胶原蛋白基因失活或突变将准备研究 XVI 型胶原蛋白的体内功能。 重组 XVI 型胶原蛋白 链将准备好研究它们与其他链的结合相互作用 细胞外基质蛋白。 XVI型转录调控 将通过启动子区域的表征来研究胶原蛋白基因 的基因。