CORE--MICROSCOPY AND IMAGING FACILITY

核心——显微镜和成像设备

• 批准号: 6203167
• 负责人: SUSETTE C MUELLER
• 金额: $ 7.93万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-30 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203167
• 关键词: bioimaging /biomedical imaging; biomedical facility; electron microscopy; light microscopy; microinjections; neoplasm /cancer

Microscopy and Imaging provides instrumentation, training, and expert advice for light and electron microscopy d image analysis. Light microscopy includes optics for bright field, dark field, phase contrast, differential interference contrast or Hoffman optics, epifluorescence wide field and confocal microscopy for high resolution light microscopy of tumor cells, tissues, and subcellular localizations. Transmission and scanning electron microscopes available for high resolution subcellular morphology. A newly acquired microinjection system allows us to microinject individual tumor cells. The inverted microscope on this system is interfaced with a camera for digitizing images. The installation of Windows-compatible Optimas 5.1 updates our capacity for image analysis. Time lapse imaging of motile cells is now underway using this work station. The shared facility has a greatly expanded capacity or image storage and transfer with the recent acquisition of writable CD-ROM and networking of the digitized imaging work stations. In addition to traditional studies supported by the core, some examples of recent projects the image analysis/ microinjection system include microinjection of plasmids and antisense oligonucleotides, quantification of nuclear size and shape, in vitro angiogenesis, live cell motility, size and extent of epithelial branching in developing mouse mammary glands, and cell surface structure. Dr. Susette Mueller is the Director and Manager of the Shared Resource, and Dr. Maozheng Dai is the Microscopist. The main laboratory of the shared source is now located on the fourth floor in the Cancer Center space within the new Research Building. The electron and confocal microscopes are m their original location in the adjacent Preclinical Science Building. The use of the facility has expanded significantly in the past three years, with about double the number of principle investigators (15 to 33), peer-reviewed projects (15 to 34), and departments (8 to 13) utilizing the Shared Resource. The Resource in-acts with all 8 programs and with 4 other Shared Resources.

显微镜和成像提供仪器，培训， 光学和电子显微镜图像分析的专家建议。 光学显微镜包括用于明场、暗场、 相位对比、微分干涉对比或霍夫曼 光学、落射荧光宽视场和共焦显微镜， 肿瘤细胞、组织的高分辨率光学显微镜检查， 亚细胞定位传输和扫描电子 显微镜可用于高分辨率的亚细胞形态。 一个新获得的显微注射系统使我们能够显微注射 单个肿瘤细胞。这个系统上的倒置显微镜是 与一个摄像头连接，用于数字化图像。安装 兼容Windows的Optimas 5.1更新了我们的图像容量 分析.运动细胞的延时成像现在正在进行， 这个工作站。共享设施大大扩展了 容量或图像存储和传输与最近采集 数字化影像工作的可写光盘化和网络化 站的除了核心支助的传统研究外， 最近的一些项目的例子，图像分析/显微注射 包括质粒和反义核酸显微注射系统 核大小和形状的定量， 体外血管生成，活细胞运动，大小和程度 发育中的小鼠乳腺上皮分支和细胞 表面结构Susette Mueller博士是董事兼经理 戴茂正博士是显微镜专家。 共享源的主要实验室现在位于 在癌症中心四楼的新研究空间内 建设电子显微镜和共聚焦显微镜是他们的 位于邻近的临床前科学大楼的原始位置。的 在过去三年中， 年，主要调查人员的数量增加了一倍（15 同行评审项目（15至34个）和部门（8至 13)利用共享资源。资源与所有8个 与其他4个共享资源。