CORE--MICROSCOPY AND IMAGING FACILITY

核心——显微镜和成像设备

• 批准号: 6203167
• 负责人: SUSETTE C MUELLER
• 金额: $ 7.93万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-30 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203167
• 关键词: bioimaging /biomedical imaging; biomedical facility; electron microscopy; light microscopy; microinjections; neoplasm /cancer

Microscopy and Imaging provides instrumentation, training, and expert advice for light and electron microscopy d image analysis. Light microscopy includes optics for bright field, dark field, phase contrast, differential interference contrast or Hoffman optics, epifluorescence wide field and confocal microscopy for high resolution light microscopy of tumor cells, tissues, and subcellular localizations. Transmission and scanning electron microscopes available for high resolution subcellular morphology. A newly acquired microinjection system allows us to microinject individual tumor cells. The inverted microscope on this system is interfaced with a camera for digitizing images. The installation of Windows-compatible Optimas 5.1 updates our capacity for image analysis. Time lapse imaging of motile cells is now underway using this work station. The shared facility has a greatly expanded capacity or image storage and transfer with the recent acquisition of writable CD-ROM and networking of the digitized imaging work stations. In addition to traditional studies supported by the core, some examples of recent projects the image analysis/ microinjection system include microinjection of plasmids and antisense oligonucleotides, quantification of nuclear size and shape, in vitro angiogenesis, live cell motility, size and extent of epithelial branching in developing mouse mammary glands, and cell surface structure. Dr. Susette Mueller is the Director and Manager of the Shared Resource, and Dr. Maozheng Dai is the Microscopist. The main laboratory of the shared source is now located on the fourth floor in the Cancer Center space within the new Research Building. The electron and confocal microscopes are m their original location in the adjacent Preclinical Science Building. The use of the facility has expanded significantly in the past three years, with about double the number of principle investigators (15 to 33), peer-reviewed projects (15 to 34), and departments (8 to 13) utilizing the Shared Resource. The Resource in-acts with all 8 programs and with 4 other Shared Resources.

显微镜和成像提供仪器，训练和 光和电子显微镜D图像分析的专家建议。 光学显微镜包括用于明亮场，深色场的光学器件， 阶段对比，差分干扰对比或霍夫曼 光学元件，荧光宽场和共聚焦显微镜高 肿瘤细胞，组织和 亚细胞定位。传输和扫描电子 可用于高分辨率亚细胞形态的显微镜。 新获得的微注射系统使我们能够微型注射 单个肿瘤细胞。该系统上的倒显微镜是 与相机连接用于数字图像。安装 与Windows兼容的Optimas 5.1更新了我们的图像能力 分析。现在正在使用动向细胞的时间衰变成像 这个工作站。共享设施大大扩展 产能或图像存储以及最近的收购 可写的CD-ROM和数字化成像工作的网络 车站。除了核心支持的传统研究外， 最近项目的一些示例图像分析/显微注射 系统包括质粒的显微注射和反义 寡核苷酸，核大小和形状的定量 体外血管生成，活细胞运动，大小和程度 发育中的小鼠乳腺和细胞中的上皮分支 表面结构。 Susette Mueller博士是董事兼经理 共享资源和Maozheng Dai博士是显微镜家。 共享来源的主要实验室现在位于 新研究的癌症中心空间的四楼 建筑。电子和共聚焦显微镜是它们的 邻近临床前科学大楼的原始位置。这 在过去的三个中，该设施的使用已大大扩展 多年，主要研究人员的数量大约是两倍（15 至33），经过同行评审的项目（15至34）和部门（8至8至 13）利用共享资源。所有8个 程序和其他4个共享资源。