Multiphoton Imaging

多光子成像

• 批准号: 7047423
• 负责人: SUSETTE C MUELLER
• 金额: $ 50万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2008-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047423
• 关键词: microscopy; neoplasm /cancer; schools; training

DESCRIPTION (provided by applicant): We request funds towards the purchase of a Zeiss510/ NLO microscope with Ti:sapphire Chameleon laser. Five principle investigators from the Medical School comprise the major user group (Faden, Mueller, Pestell, Roman, Van Keuren) and 8 minor users are described including faculty from the Medical Center and the Undergraduate Campus. A total of 11 NIH-funded investigators will be supported by this application. Projects conducted by major users include: vital imaging of invasion in tissues by tumor or transgenic mammary epithelial cells; vital imaging in situ of mitochondrial function in liver; imaging temperature sensitive probes in skin; and imaging vasculogenesis in zebrafish. Minor projects include imaging the effects of stable chemotactic gradients on neuronal growth cones embedded in thick collagen gels; vital imaging of neurons and parasite infection in the gut; trafficking of G protein-coupled receptors in hypertension; live imaging of vacuolar pH within living malarial parasites; and supporting the research and educational mission of the Lombardi Comprehensive Cancer Center (LCCC) Microscopy and Imaging Shared Resource (MISR). The new microscope will be housed in the LCCC MISR. The Principle Investigator, Dr. Mueller, is an NIH funded tumor cell biologist and has been director of this facility since 1991. LCCC and Georgetown University have committed space, additional funding to meet the total purchase price and yearly service contract dollars to support the acquisition and maintenance of this instrument. Members of the major user group with microscopy expertise including the PI (Mueller) and a physicist with multiphoton expertise (Van Keuren) will be trained initially together with one of our full time MISR microscopists (Palchik). Mr. Palchik who has expertise in confocal and digital light microscopy will support use and training under the direction of Drs. Mueller and Van Keuren. The shared resource will perform associated administrative tasks including billing. Oversight will be provided by our 8 member advisory committee. In 2004, the Shared Resource was utilized by 60 investigators representing 15 departments in the Medical School and the Undergraduate Campus. MISR has an outstanding record in training. Each year since 1999, we have trained between 30- 40 LCC investigators to use the existing microscopes in the MISR. The Shared Resource is maintained by a combination of Cancer Center and grant support. Users contribute to the resource in the form of user charge back fees. There is no multiphoton microscope at Georgetown and animal imaging activities are severely curtailed as a result.

描述(由申请人提供)： 我们请求资金用于购买带有钛宝石变色龙激光器的Zeiss510/NLO显微镜。描述了来自医学院的5名主要调查人员组成的主要用户组(Faden，Mueller，Pestell，Roman，Van Keuren)和8名次要用户，包括来自医学中心和本科生校园的教职员工。这项申请将支持总共11名NIH资助的调查人员。主要用户开展的项目包括：肿瘤或转基因乳腺上皮细胞侵入组织的生命成像；肝脏线粒体功能的原位生命成像；皮肤温度敏感探针的成像；斑马鱼血管生成的成像。小项目包括成像稳定的化学趋化梯度对嵌入厚胶原胶原凝胶的神经元生长锥体的影响；神经元和肠道寄生虫感染的生命成像；G蛋白偶联受体在高血压中的传输；活的疟疾寄生虫内空泡pH的实时成像；以及支持伦巴迪综合癌症中心(LCCC)显微镜和成像共享资源(MISR)的研究和教育任务。新的显微镜将被安置在LCCC MISR中。首席调查员米勒博士是美国国立卫生研究院资助的肿瘤细胞生物学家，自1991年以来一直担任该机构的主任。LCCC和乔治敦大学已经承诺提供空间、额外资金以满足总购买价格和年度服务合同美元，以支持该仪器的购买和维护。拥有显微镜专业知识的主要用户群体的成员，包括PI(Mueller)和一名具有多光子专业知识的物理学家(Van Keuren)，最初将与我们的一名MISR全职显微镜专家(Palchik)一起接受培训。帕尔奇克在共焦显微镜和数字光学显微镜方面拥有专业知识，他将在穆勒和范·凯伦博士的指导下支持使用和培训。共享资源将执行相关的管理任务，包括记账。监督将由我们的8人咨询委员会提供。2004年，代表医学院和本科生校园15个科室的60名调查人员使用了共享资源。MISR在训练中有着出色的记录。自1999年以来，我们每年培训30-40名LCC调查人员使用MISR现有的显微镜。共享资源由癌症中心和赠款支持相结合来维护。用户以用户收取费用的形式对资源进行贡献。乔治敦大学没有多光子显微镜，动物成像活动因此受到严重限制。