MULTIPHOTON IMAGING

多光子成像

• 批准号: 7334975
• 负责人: SUSETTE C MUELLER
• 金额: $ 50万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334975
• 关键词: microscopy; neoplasm /cancer; schools; training

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We request funds towards the purchase of a Zeiss510/ NLO microscope with Ti:sapphire Chameleon laser. Five principle investigators from the Medical School comprise the major user group (Faden, Mueller, Pestell, Roman, Van Keuren) and 8 minor users are described including faculty from the Medical Center and the Undergraduate Campus. A total of 11 NIH-funded investigators will be supported by this application. Projects conducted by major users include: vital imaging of invasion in tissues by tumor or transgenic mammary epithelial cells; vital imaging in situ of mitochondrial function in liver; imaging temperature sensitive probes in skin; and imaging vasculogenesis in zebrafish. Minor projects include imaging the effects of stable chemotactic gradients on neuronal growth cones embedded in thick collagen gels; vital imaging of neurons and parasite infection in the gut; trafficking of G protein-coupled receptors in hypertension; live imaging of vacuolar pH within living malarial parasites; and supporting the research and educational mission of the Lombardi Comprehensive Cancer Center (LCCC) Microscopy and Imaging Shared Resource (MISR). The new microscope will be housed in the LCCC MISR. The Principle Investigator, Dr. Mueller, is an NIH funded tumor cell biologist and has been director of this facility since 1991. LCCC and Georgetown University have committed space, additional funding to meet the total purchase price and yearly service contract dollars to support the acquisition and maintenance of this instrument. Members of the major user group with microscopy expertise including the PI (Mueller) and a physicist with multiphoton expertise (Van Keuren) will be trained initially together with one of our full time MISR microscopists (Palchik). Mr. Palchik who has expertise in confocal and digital light microscopy will support use and training under the direction of Drs. Mueller and Van Keuren. The shared resource will perform associated administrative tasks including billing. Oversight will be provided by our 8 member advisory committee. In 2004, the Shared Resource was utilized by 60 investigators representing 15 departments in the Medical School and the Undergraduate Campus. MISR has an outstanding record in training. Each year since 1999, we have trained between 30- 40 LCC investigators to use the existing microscopes in the MISR. The Shared Resource is maintained by a combination of Cancer Center and grant support. Users contribute to the resource in the form of user charge back fees. There is no multiphoton microscope at Georgetown and animal imaging activities are severely curtailed as a result.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）： 我们申请资金用于购买带钛宝石变色龙激光器的Zeiss 510/ NLO显微镜。来自医学院的五名主要研究者组成了主要用户组（Faden、Mueller、Pestell、Roman、货车Keuren），描述了8名次要用户，包括来自医学中心和本科校园的教师。共有11名NIH资助的研究人员将得到该应用程序的支持。主要用户开展的项目包括：肿瘤或转基因乳腺上皮细胞侵入组织的活体成像;肝脏线粒体功能原位活体成像;皮肤温度敏感探针成像;斑马鱼血管发生成像。次要项目包括成像稳定的趋化梯度对嵌入厚胶原凝胶中的神经元生长锥的影响;神经元和肠道寄生虫感染的生命成像;高血压中G蛋白偶联受体的运输;活疟疾寄生虫内空泡pH值的实时成像;并支持伦巴第综合癌症中心（LCCC）显微镜和成像共享资源（MISR）的研究和教育使命。新的显微镜将被安置在LCCC MISR中。主要研究者Mueller博士是NIH资助的肿瘤细胞生物学家，自1991年以来一直担任该机构的主任。LCCC和乔治敦大学已承诺空间，额外的资金，以满足总购买价格和每年的服务合同美元，以支持购买和维护这一仪器。具有显微镜专业知识的主要用户组成员，包括PI（Mueller）和具有多光子专业知识的物理学家（货车Keuren），最初将与我们的全职MISR显微镜专家（Palchik）一起接受培训。Palchik先生拥有共聚焦和数字光学显微镜方面的专业知识，将在Mueller和货车Keuren博士的指导下支持使用和培训。共享资源将执行相关的管理任务，包括计费。我们将由8名成员组成的咨询委员会进行监督。在2004年，共享资源被60名调查员代表在医学院和本科校园的15个部门使用。MISR在培训方面有着出色的记录。自1999年以来，我们每年都培训30- 40名LCC研究人员使用MISR中现有的显微镜。共享资源由癌症中心和赠款支持的组合维护。用户以用户收费的形式贡献资源。乔治敦大学没有多光子显微镜，因此动物成像活动受到严重限制。