Structural biology

描述：(申请者描述)结构生物学课程是癌症中心的一个新成员，是自然融合的结果。1癌症研究中结构信息的必要性；2结构生物学家专家组的招募和弗吉尼亚大学结构生物学中心的建立；3这些研究人员的许多科学兴趣的癌症焦点，包括多药耐药P-糖蛋白、RAS癌基因、小GTP酶、RhoA。和磷酸酪氨酸结合接头蛋白Shc。该计划的总体目标是通过跨学科合作，整合肿瘤发生、转移以及最终癌症治疗的结构和功能研究。现有的专业知识和方法包括X射线结晶学、核磁共振和电子顺磁共振光谱、原子力和冷原子力显微镜、全内反射荧光显微镜、激光扫描共聚焦显微镜、X射线测绘、能量过滤扫描电子显微镜和电子探针X射线显微分析。在该计划成立后的短时间内(1998年5月)，我们在肿瘤相关蛋白的高分辨结构测定(Small GTPase-FAK)方面取得了重大进展，并启动了试点项目(多药耐药糖蛋白P)。该计划还通过由国际知名的结构生物学家参加的一系列研讨会来传播结构方法的价值和使用。

Description: (Applicant's Description) The Structural Biology Program is a new addition to the Cancer Center and resulted from the natural convergence of. 1 The necessity for structural information in cancer research; 2 The recruitment of an expert group of structural biologists and establishment of a Center for Structural Biology at the University of Virginia and; 3 The cancer-focus of many of the scientific interests of these investigators, including the multi-drug resistance P-glycoprotein, the Ras oncogene, the small GTPase, RhoA. and the phosphotyrosine-binding adapter protein, Shc. The overall goal of the Program is to integrate, through interdisciplinary collaborations, structural with functional studies of oncogenesis, metastasis and, ultimately, cancer therapy. Expertise and methodologies available include X-ray crystallography, NMR and EPR spectroscopy, atomic force and cryo-atomic force microscopy, total internal reflectance fluorescence microscopy, laser scanning confocal microscopy, X-ray mapping, energy filtered scanning electron microscopy and electron probe X-ray microanalysis. In the short time since the Program was established (May 1998) we made significant advances in high resolution structure determination of cancer related proteins (small GTPase-FAK), and initiated pilot projects (multi-drug resistance glycoprotein P). The Program also disseminates the value and use of structural methods through a seminar series featuring internationally renowned structural biologists.