GENETIC ANALYSIS OF B3 ADRENERGIC RECEPTOR FUNCTION

β 3-肾上腺素能受体（B3-AR）被认为是重要的 调节能量消耗和B3选择性激动剂的作用， 被开发成减肥药。 许多尚未解决的问题 坚持认为这种受体作为抗肥胖的潜力 目标 在以前的研究中已经产生了缺乏B3-AR的敲除小鼠。 补助期。 本申请提出1）使用缺乏 评估B3-AR在调节能量平衡中的作用： 饮食和背景基因的影响2）评估 基因改变的a2-AR/B3 AR比率或Trp 64 Arg人B3-AR 脂肪细胞中肾上腺素能信号传导的变体; 3）确定 人B3-AR基因在棕色人种中选择性表达的分子基础 脂肪细胞; 4）使用缺乏B3-AR的小鼠来建立 额外的非典型B-AR，并进行分子克隆和表征 这些新的受体。

Beta 3-adrenergic receptors (B3-AR) are thought to play an important role in regulating energy expenditure and B3-selective agonists are being developed as anti-obesity drugs. A number of unresolved issues persist regarding the potential of this receptor as an anti-obesity target. Knockout mice lacking B3-AR have been generated in the previous grant period. The present application proposed to 1) use mice lacking B3-ARs to assess the role of B3-ARs in regulating energy balance: influence of diet and background genes 2) to assess effects of genetically altered a2-AR/B3AR ratios or the Trp64Arg human B3-AR variant on adrenergic signalling in adipocytes; 3) to determine the molecular basis for selective expression of human B3-ARs in brown adipocytes; 4) to use mice lacking B3-AR to establish the existence of additional atypical B-ARs and to molecularly clone and characterize these novel receptors.