Transgenic Core

BADERC转基因核心位于贝丝以色列女执事医疗中心(BIDMC)。核心提供胚胎显微注射服务；具体地说，显微注射研究人员衍生的转基因DNA结构，以制造转基因小鼠，以及显微注射研究人员衍生的靶向胚胎干细胞(ES细胞)，目的是制造基因敲除小鼠或基因敲除小鼠(即定位等位基因、点突变等)。在这笔赠款的最后5年周期中(2004-2009年)，BADERC转基因核心提供了及时的、具有成本效益的服务。总共执行了92个项目(注射DNA转基因-49个项目，注射靶向ES细胞克隆-40个项目，突变胚胎的周期保存-3个项目)。这一服务水平比这笔赠款的前五年周期(1999-2004年)增加了84%。这些服务是向来自4个不同机构的15名不同的BADERC调查人员提供的。在此期间，BADERC调查人员发表了43篇论文，涉及利用BIDMC转基因设施产生的转基因小鼠。此外，BADERC 研究人员获得了22项基于美国国立卫生研究院的资助，涉及基因工程小鼠的生成。总而言之，转基因核心已经为BADERC的各种富有成效的研究人员提供了广泛的、具有成本效益的服务。

The BADERC Transgenic Core is located at the Beth Israel Deaconess Medical Center (BIDMC). The Core performs embryo microinjection services; specifically, microinjection of investigator-derived transgenic DNA constructs, for the purpose of making transgenic mice, and also microinjection of investigator-derived targeted embryonic stem cells (ES cells), for the purpose of making gene knockout mice or gene knockin mice (i.e. loxed alleles, point mutations, etc.). In the last 5 year cycle of this grant (2004-2009), the BADERC Transgenic Core provided timely, cost-effective services. In total, 92 projects were performed (injection of DNA transgenes - 49 projects, injection of targeted ES cell clones - 40 projects, cyropreservation of mutant embryos - 3 projects). This level of services represents a 84% increase in activity over the previous 5 year cycle of this grant (1999-2004). These services were provided to 15 different BADERC investigators from 4 different institutions. During this period, BADERC investigators published 43 papers that involved the utilization of genetically altered mice generated by the BIDMC Transgenic Facility. In addition, BADERC investigators obtained 22 NIH-based grants that involved the generation of genetically engineered mice. In summary, the Transgenic Core has provided extensive, cost-effective services to a wide array of productive BADERC investigators.