RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU

描述（改编自申请人的摘要）：在本申请中， 私家侦探提出了一系列旨在解决本地化问题的目标， 酵母细胞中的ASH1 mRNA。 Ash1p是一种不对称定位蛋白， 在抑制子细胞中交配类型转换方面很重要。 的 这种核蛋白的不对称性是由其mRNA的定位引起的 在子细胞的远端皮层，芽，和更大的 这种基因表达的负调节因子在子代中的积累 原子核。 在第一个目标中，顺式作用序列和反式作用因子 将被分离，以确定蛋白质组分的 机制 具体目标2提出了跟踪运输的实验， 将该RNA从其转录位点输出并定位到 它的最终位置在芽的皮层。 在最后的具体目标中， 将对核出口机制进行评估， 可能存在速率限制的路径。

DESCRIPTION (Adapted from the applicant's abstract): In this application, the P.I. proposes a series of aims designed to address the localization of ASH1 mRNA in yeast cells. Ash1p is an asymmetrically localized protein, important in repressing mating type switching in the daughter cell. The asymmetry of this nuclear protein results from the localization of its mRNA at the distal cortex of a daughter cell, the bud, and the greater accumulation of this negative regulator of gene expression in daughter nuclei. In the first aim, cis-acting sequences and trans-acting factors will be isolated in order to determine the protein components of the mechanism. Specific aim 2 proposes experiments to follow the transport, export and localization of this RNA from the site of its transcription to its final location at the cortex of the bud. In the final specific aim, the mechanism of nuclear export will be assessed to determine points in the pathway where rate-limitations may exist.