MICROTUBULE BASED TRANSPORT IN EARLY EMBRYOS

早期胚胎中基于微管的运输

• 批准号: 6019261
• 负责人: Jonathan M. Scholey
• 金额: $ 24.87万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019261
• 关键词: MICROTUBULE; BASED; TRANSPORT; EARLY; EMBRYOS

DESCRIPTION: This application is focused on the analysis of mechanisms and functions of MT-based motors that the investigators postulate drive specific transport event that occurs during early development. They propose to : 1) characterize the structure, function and domain organization of a putative minus-end-directed kinesin holoenzyme and to analyze its role in nuclear transport and mitosis; 2) to further analyze structure-function relationships in the morphogenesis and function of mitotic spindles; 3) to biochemically characterize the vesicular cargo of the sea urchin embryonic mitotic spindle for delivery to different destinations and 4) to purify the Drosophila Nod chromosomal kinesin-like motor from transgenic fly lines, in order to analyze the mechanism by which it transports chromosomes along MTs.

描述：本应用侧重于机理分析和