MECHANISMS OF HOST RESISTANCE TO GROUP B STREPTOCOCCI

描述(改编自申请人的摘要)：目前的提案将 探讨细胞因子在B组链球菌(GBS)应答中的作用 在体外和体内的实验模型中。研究的目的是 检查肿瘤坏死因子α的连续出现情况。(TNFa)， 人体外暴露后IL-1β、IL-6和IL-8的变化 单个核细胞转化为GBS。这些细胞因子的顺序出现将 在新生大鼠接种GBS后也进行了评估。我们会 通过关键的RGD序列研究纤维连接蛋白(FN)在 辐射对人单个核细胞释放肿瘤坏死因子α的诱导或促进作用 B组链球菌。C5a与GBS一起作为辅助因子在 此外，还将探索在体外导致细胞因子释放的问题。这些研究将 包括旨在确定GBS C5a-ASE是否会影响C5a的实验 诱导或促进TNFa的释放。这些研究应该会产生数据 这将有助于解释促炎细胞因子在脑出血中的作用。 这种最暴发性新生儿感染性休克的发病机制 细菌感染。

DESCRIPTION (Adapted from applicant's abstract): The current proposal will explore the role of cytokines in the response to group B streptococci (GBS) in vitro and in vivo in an experimental model. Studies are designed to examine the sequential appearance of tumor necrosis factor alpha. (TNFa), IL-1 beta (IL-1b), IL-6, and IL-8 following in vitro exposure of human mononuclear cells to GBS. The sequential appearance of these cytokines will also be assessed following GBS inoculation of neonatal rats. We will examine the role of fibronectin (FN) through the critical RGD sequence, in inducing or enhancing TNFa release by human mononuclear cells exposed to group B streptococci. The role of C5a as a co-factor along with GBS in causing cytokine release in vitro will also be explored. These studies will include experiments designed to determine if the GBS C5a-ase can affect C5a induction or enhancement of TNFa release. These studies should yield data that will help to explain the role of the proinflammatory cytokines in the pathogenesis of septic shock due to this most fulminant form of neonatal bacterial infection.