CORNEAL INFLAMMATION AND INTERLEUKIN 10

角膜炎症和白细胞介素 10

• 批准号: 2888518
• 负责人: ROBERT N LAUSCH
• 金额: $ 25.52万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2888518
• 关键词: animal tissue; cell cell interaction; cornea disorder; corneal epithelium; cytokine receptors; fibroblasts; flow cytometry; human T cell leukemia; immunocytochemistry; immunoregulation; inflammation; interleukin 10; laboratory mouse; polymerase chain reaction; receptor binding

DESCRIPTION (Adapted from applicant's abstract): The focus of this project is to investigate resident corneal cell interaction with the immunoregulatory cytokine IL-10. Studies will be carried out with cultured human and murine corneal epithelial cells and fibroblasts. RT- PCR analysis will be used to determine precisely which resident corneal cells express IL-10 receptor mRNA. Flow cytometry and immunohistology will be used to search for the protein product on the different corneal cell types. The consequences of IL-10 binding to its receptor on corneal cells will be analyzed from the perspective of determining whether ligand binding leads to suppressed cytokine/chemokine mRNA and/or protein synthesis. Prior RT-PCR and ELISA studies in our laboratory have shown that IL-10 suppresses IL-6 mRNA and protein synthesis. The applicant hypothesizes that IL-10 inhibits cytokine synthesis at the level of gene transcription or interferes with mRNA stability. Preliminary data indicate that human and mouse corneas possess mRNA for IL-10. The applicant will test whether resident corneal cells make IL-10 protein. Positive results would suggest that IL-10 may function as an autoregulatory factor for resident corneal cells. The basic information generated by these studies may prove useful for devising new approaches to control ocular inflammation.

描述（改编自申请人的摘要）：本文的重点 该项目旨在研究常驻角膜细胞与 免疫调节细胞因子IL-10。 研究将与 培养的人和鼠角膜上皮细胞和成纤维细胞。 RT- PCR分析将用于精确确定哪种常驻角膜 细胞表达IL-10受体mRNA。 流式细胞术和免疫组织学 将被用于搜索不同角膜上的蛋白质产物 细胞类型。 白细胞介素-10与其受体结合对角膜上皮细胞的影响 细胞将从确定是否 配体结合导致抑制的细胞因子/趋化因子mRNA和/或 蛋白质合成 我们实验室先前的RT-PCR和ELISA研究 显示IL-10抑制IL-6 mRNA和蛋白质合成。 的 申请人假设IL-10抑制细胞因子合成， 基因转录水平或干扰mRNA稳定性。 初步数据表明，人类和小鼠角膜具有mRNA， IL-10。 申请人将测试常驻角膜细胞是否使 IL-10蛋白。 阳性结果表明IL-10可能在 作为常驻角膜细胞的自动调节因子。 基本 这些研究产生的信息可能有助于设计新的 控制眼部炎症的方法。