CONJUNCTIVAL MUCINS--EXPRESSION AND SECRETION

虽然粘蛋白糖蛋白是前体细胞的主要功能成分， 眼泪膜，很少有人知道他们的分子身份， 合成或分泌。人肠道呼吸系统的分子研究 和乳腺粘蛋白导致了 八种不同的粘蛋白种类（MUC 1-MUC 8）。不同粘膜组织 具有独特的保护要求，这些要求通过组织特异性 粘蛋白表达和/或分泌的差异。的总体目标 建议的研究是确定结膜粘蛋白的贡献， 人类的泪膜要研究的假设是， 粘蛋白由几种不同的基因产物组成， 可以进行不同的调节。我们建议使用分子生物学， 免疫学、形态学和药理学方法来研究 正常人结膜粘蛋白基因的表达和分泌。目标 该项目的主要内容是： 1.人结膜组织中粘蛋白基因产物的鉴定。 2.结膜粘蛋白基因区域和细胞位点的定位 表情 3.眼前泪膜粘蛋白成分及分泌分析。 拟议中的研究将是任何系统中最先探索的研究之一， 粘蛋白基因表达与分泌的关系。 控制合成、储存和代谢的生物化学途径的研究 粘蛋白的释放可能提出了新的分子方法来控制 眼粘蛋白产生。

Although mucin glycoproteins are major functional constituents of the pre- ocular tear film, little is known about their molecular identity, synthesis or secretion. Molecular studies of human intestinal respiratory and mammary mucins have led to the identification and partial sequencing of eight distinct mucin species (MUCl-MUC8). Different mucosal tissues have unique protective requirements which are reflected by tissue specific differences in mucin expression and/or secretion. The overall goal of the proposed studies is to define the contribution of conjunctival mucins to the human tear film. The hypothesis to be investigated is that ocular mucins consist of several distinct gene products the secretion of which may be differentially regulated. We propose to use molecular biological, immunological, morphological and pharmacological approaches to investigate mucins gene expression and secretion in normal human conjunctiva. The Aims of this project are: 1. Identification of mucin gene products in human conjunctival tissue. 2. Localization of regional and cellular sites of conjunctival mucin gene expression. 3. Analysis of mucin composition and secretion in the preocular tear film. The proposed studies will be among the first, in any system, to explore the relationship between mucin gene expression and secretion. Investigation of biochemical pathways governing synthesis, storage and release of mucin may suggest novel molecular approaches to control of ocular mucin production.