CONJUNCTIVAL MUCINS--EXPRESSION AND SECRETION

结膜粘蛋白——表达和分泌

• 批准号: 2888451
• 负责人: MARCIA M JUMBLATT
• 金额: $ 15.76万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2888451
• 关键词: RNase protection assay; SDS polyacrylamide gel electrophoresis; age difference; conjunctiva; enzyme linked immunosorbent assay; gender difference; gene expression; glycoprotein biosynthesis; human genetic material tag; human tissue; in situ hybridization; messenger RNA; molecular cloning; molecular site; mucins; muscarinic receptor; northern blottings; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; protein structure; secretion; tear; ultrafiltration

Although mucin glycoproteins are major functional constituents of the pre- ocular tear film, little is known about their molecular identity, synthesis or secretion. Molecular studies of human intestinal respiratory and mammary mucins have led to the identification and partial sequencing of eight distinct mucin species (MUCl-MUC8). Different mucosal tissues have unique protective requirements which are reflected by tissue specific differences in mucin expression and/or secretion. The overall goal of the proposed studies is to define the contribution of conjunctival mucins to the human tear film. The hypothesis to be investigated is that ocular mucins consist of several distinct gene products the secretion of which may be differentially regulated. We propose to use molecular biological, immunological, morphological and pharmacological approaches to investigate mucins gene expression and secretion in normal human conjunctiva. The Aims of this project are: 1. Identification of mucin gene products in human conjunctival tissue. 2. Localization of regional and cellular sites of conjunctival mucin gene expression. 3. Analysis of mucin composition and secretion in the preocular tear film. The proposed studies will be among the first, in any system, to explore the relationship between mucin gene expression and secretion. Investigation of biochemical pathways governing synthesis, storage and release of mucin may suggest novel molecular approaches to control of ocular mucin production.

虽然粘蛋白糖蛋白是前-的主要功能成分