CONJUNCTIVAL MUCINS--EXPRESSION AND SECRETION

结膜粘蛋白——表达和分泌

• 批准号: 2415029
• 负责人: MARCIA M JUMBLATT
• 金额: $ 14.57万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415029
• 关键词: RNase protection assay; SDS polyacrylamide gel electrophoresis; age difference; conjunctiva; enzyme linked immunosorbent assay; gender difference; gene expression; glycoprotein biosynthesis; human genetic material tag; human tissue; in situ hybridization; messenger RNA; molecular cloning; molecular site; mucins; muscarinic receptor; northern blottings; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; protein structure; secretion; tear; ultrafiltration

Although mucin glycoproteins are major functional constituents of the pre- ocular tear film, little is known about their molecular identity, synthesis or secretion. Molecular studies of human intestinal respiratory and mammary mucins have led to the identification and partial sequencing of eight distinct mucin species (MUCl-MUC8). Different mucosal tissues have unique protective requirements which are reflected by tissue specific differences in mucin expression and/or secretion. The overall goal of the proposed studies is to define the contribution of conjunctival mucins to the human tear film. The hypothesis to be investigated is that ocular mucins consist of several distinct gene products the secretion of which may be differentially regulated. We propose to use molecular biological, immunological, morphological and pharmacological approaches to investigate mucins gene expression and secretion in normal human conjunctiva. The Aims of this project are: 1. Identification of mucin gene products in human conjunctival tissue. 2. Localization of regional and cellular sites of conjunctival mucin gene expression. 3. Analysis of mucin composition and secretion in the preocular tear film. The proposed studies will be among the first, in any system, to explore the relationship between mucin gene expression and secretion. Investigation of biochemical pathways governing synthesis, storage and release of mucin may suggest novel molecular approaches to control of ocular mucin production.

尽管粘蛋白糖蛋白是Pre-Pre的主要功能成分 眼泪膜，人们对它们的分子身份知之甚少， 合成或分泌。人体肠道呼吸系统的分子生物学研究 和乳腺粘蛋白导致了鉴定和部分测序 8种不同的粘蛋白(MUCL-MUC8)。不同的粘膜组织 具有独特的保护要求，这些要求由组织特异性反映 粘蛋白表达和/或分泌的差异。该计划的总体目标 建议的研究是确定结膜粘蛋白对 人类的泪膜。要调查的假设是，眼睛 粘蛋白由几种不同的基因产物组成，它们的分泌 可能受到不同的监管。我们建议使用分子生物学， 免疫学、形态学和药理学的研究方法 粘蛋白基因在正常人结膜中的表达和分泌。目标 该项目的主要内容包括： 1.人结膜组织中粘蛋白基因产物的鉴定 2.结膜粘蛋白基因的区域和细胞定位 表情。 3.眼前泪膜粘蛋白组成和分泌的分析。 拟议中的研究将是任何系统中最早探索的研究之一 粘蛋白基因表达与分泌的关系。 生物化学途径控制合成、储存和 粘蛋白的释放可能会提示控制肺炎的新的分子方法 眼部粘蛋白的产生。