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BINDING AND MEMBRANE ASSOCIATION OF RETINA

视网膜结合和膜协会

基本信息

批准号：
6017474
负责人：
ROBERT E HAUSMAN
金额：
$ 0.72万
依托单位：
BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
依托单位国家：
美国
项目类别：
财政年份：
1995
资助国家：
美国
起止时间：
1995-01-01 至 1999-12-31
项目状态：
已结题

项目摘要

We are interested in the position signaling in the developing nervous system that is responsible for terminal differentiation of neurons. Specifically, we are interested in the roles of a vertebrate retinal cell recognition protein (R-cognin) and those proteins which interact with R- cognin. Is the R-cognin complex at the cell surface a cell signaling mechanism that helps determine the differentiation pathway taken by the embryonic chick retinal neurons? The chick retina provides a useful model system for this because it is large, easily accessible and avascular. We have four specific aims. 1) To confirm the relationship between the chicken multifunctional proteins and R-cognin. 2) To determine the sequence of the R-cognin protein. 3) To determine how R-cognin protein is associated with the retina cell surface. It is already clear that R-cognin has a unique cell surface location in retina tissue, which is likely to be critical for our understanding of how antibody to R-cognin affects developmental increases in GAD and ChAT activity in retina. 4) To determine the cell types in the developing retina that display and synthesize R-cognin. By investigating one specific molecule underlying neurogenesis in retina, we seek a basic understanding of neuronal determination. This is basic research on the establishment and stability of the differentiated state; however, it will help us understand the capacity of the retina for regeneration and provide clues about how abnormal decisions in embryogenesis or early postnatal life might affect the ability of retina to subsequently respond to trauma or which might underlie pathologies in vision that appear later in life.

我们对神经发育中的位置信号感兴趣负责神经元终末分化的系统。具体来说，我们对脊椎动物视网膜细胞的作用感兴趣识别蛋白（R-cognin）和那些与 R-相互作用的蛋白质科宁。细胞表面的 R-cognin 复合物是细胞信号传导吗有助于确定分化途径的机制胚胎鸡视网膜神经元？小鸡视网膜提供了一个有用的模型系统之所以如此，是因为它庞大、易于接近且无血管。我们有四个具体目标。 1）确认之间的关系鸡多功能蛋白和R-cognin。 2）确定 R-cognin 蛋白的序列。 3) 确定R-cognin蛋白的性质与视网膜细胞表面有关。已经很清楚，R-cognin 在视网膜组织中具有独特的细胞表面位置，这可能是对于我们了解 R-cognin 抗体如何影响至关重要视网膜中 GAD 和 ChAT 活性的发育增加。 4) 至确定发育中视网膜中显示和显示的细胞类型合成R-cognin。通过研究潜在的一种特定分子视网膜的神经发生，我们寻求对神经元的基本了解决心。这是建立和稳定性的基础研究分化状态；然而，它将帮助我们理解视网膜的再生能力并提供有关如何再生的线索胚胎发生或出生后早期生活的异常决定可能会影响视网膜随后对创伤做出反应的能力或可能是晚年出现的视力病变的基础。