BINDING AND MEMBRANE ASSOCIATION OF RETINA

视网膜结合和膜协会

• 批准号: 2019966
• 负责人: ROBERT E HAUSMAN
• 金额: $ 15.09万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2019966
• 关键词: binding proteins; biological signal transduction; cell differentiation; cell membrane; cell type; chemical binding; chick embryo; developmental neurobiology; embryogenesis; immunocytochemistry; immunofluorescence technique; in situ hybridization; laboratory rabbit; membrane proteins; neurogenesis; polymerase chain reaction; protein sequence; protein structure; retina; tissue /cell culture; vertebrate embryology; western blottings

We are interested in the position signaling in the developing nervous system that is responsible for terminal differentiation of neurons. Specifically, we are interested in the roles of a vertebrate retinal cell recognition protein (R-cognin) and those proteins which interact with R- cognin. Is the R-cognin complex at the cell surface a cell signaling mechanism that helps determine the differentiation pathway taken by the embryonic chick retinal neurons? The chick retina provides a useful model system for this because it is large, easily accessible and avascular. We have four specific aims. 1) To confirm the relationship between the chicken multifunctional proteins and R-cognin. 2) To determine the sequence of the R-cognin protein. 3) To determine how R-cognin protein is associated with the retina cell surface. It is already clear that R-cognin has a unique cell surface location in retina tissue, which is likely to be critical for our understanding of how antibody to R-cognin affects developmental increases in GAD and ChAT activity in retina. 4) To determine the cell types in the developing retina that display and synthesize R-cognin. By investigating one specific molecule underlying neurogenesis in retina, we seek a basic understanding of neuronal determination. This is basic research on the establishment and stability of the differentiated state; however, it will help us understand the capacity of the retina for regeneration and provide clues about how abnormal decisions in embryogenesis or early postnatal life might affect the ability of retina to subsequently respond to trauma or which might underlie pathologies in vision that appear later in life.

我们感兴趣的是发育中的神经元中的位置信号 负责神经元终末分化的系统。 具体来说，我们感兴趣的是脊椎动物视网膜细胞的作用， 识别蛋白（R-认知蛋白）和那些与R-认知蛋白相互作用的蛋白质。 科宁细胞表面的R-认知蛋白复合物是细胞信号传导吗 这一机制有助于确定细胞所采取的分化途径， 鸡胚视网膜神经元小鸡的视网膜提供了一个有用的模型 系统，因为它是大的，容易接近和无血管。我们 有四个具体目标。1)为了确认 鸡多功能蛋白和R-同源蛋白。 2)确定 R-cognin蛋白的序列。3)为了确定R-认知蛋白是如何 与视网膜细胞表面相关。很明显，R-认知 在视网膜组织中有一个独特的细胞表面位置， 对于我们理解抗R-认知蛋白抗体如何影响 视网膜中GAD和ChAT活性的发育性增加。4)到 确定发育中的视网膜中显示和 合成R-cognin。通过研究一种特定的分子 在视网膜神经发生，我们寻求一个基本的了解神经元 保持战略定力这是建立和稳定的基础性研究 但是，这将有助于我们理解 视网膜再生的能力，并提供线索， 胚胎发育或出生后早期的异常决定可能会影响 视网膜随后对创伤作出反应能力，或者可能 是日后出现的视觉疾病的基础。