GENETIC TOXICITY TESTING IN RODENTS

啮齿动物遗传毒性测试

• 批准号: 2800414
• 负责人: RAYMOND R TICE
• 金额: $ 19.61万
• 依托单位: INTEGRATED LABORATORY SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2800414
• 关键词: DNA damage; carcinogen testing; environmental toxicology; genetically modified animals; laboratory mouse; laboratory rat; mutagen testing; toxicant screening; toxicology information system

The objective of this project is the development of in vivo genetic toxicity data for the Environmental Toxicology Program (ETP) of the NTP. This work is achieved by in-house research programs and contract research, development, and testing programs. The contact obtained under this solicitation would allow for the testing of approximately 15 chemicals per year for 5 years for somatic cell genetic effects in rats and/or mice. Tests to be performed will include: chromosome aberrations (ABS) in bone marrow and peripheral lymphocytes; micronuclei (MN) in bone marrow, peripheral erythrocytes from sub-chronic studies (mice only), and other tissues (e.g., peripheral lymphocytes, lung, spleen); centromeric probe analysis of MN;single-cell gel electrophoresis assays of various tissues; and measurement of mutations in liver and other tissues of transgenic animals. Not all tests will be performed on all chemicals. The chemicals tested will be selected from among those that have undergone subchronic and chronic animal toxicity and/or carcinogenicity testing, or that are proposed for such testing, or are of special interest to the NTP. The results obtained from this contract will be used for setting priorities for long-term animal testing, and will provide information on mechanisms of action to support the interpretations of long-term animal toxicity results. Completion of these studies will also allow for the further development of the ETP's genetic toxicity database which will be used to determine the level of agreement between in vivo and in vitro genetic toxicity tests and the predictivity of in vivo results for rodent carcinogenesis.

本项目的目标是体内遗传学的发展。 NTP环境毒理学项目(ETP)的毒性数据。 这项工作是通过内部研究计划和合同研究实现的， 开发和测试程序。在此情况下获得的联系人 征集将允许对大约15种化学品进行测试 对大鼠和/或小鼠的体细胞遗传效应进行为期5年的研究。 将进行的测试将包括：骨骼中的染色体畸变(ABS) 骨髓和外周淋巴细胞；骨髓微核(MN)； 来自亚慢性研究的外周红细胞(仅限小鼠)，以及其他 组织(如外周淋巴细胞、肺、脾)；着丝粒探针 MN的分析；各种组织的单细胞凝胶电泳法； 并检测转基因动物肝脏和其他组织中的突变 动物。并不是所有的测试都会在所有的化学品上进行。 测试的化学物质将从经历过的化学物质中挑选出来 亚慢性和慢性动物毒性和/或致癌性测试；或 被提议用于此类测试，或对NTP特别感兴趣的。 从本合同中获得的结果将用于制定 长期动物试验的优先事项，并将提供关于 支持对长期动物的解释的作用机制 毒性结果。这些研究的完成还将使 进一步开发ETP的遗传毒性数据库，该数据库将 用于确定体内和体外之间的一致性水平 啮齿动物遗传毒性试验及其体内结果的预测性 致癌。