CORE--SCIENTIFIC RESOURCE

科学资源中心负责开展各种 科学程序，提供数据和组织样本， 进行全面的数据管理， 统计分析 该核心的具体功能如下： （1）以平衡的方式将动物分配给三个 不同的灌注组，以确保年龄分布均匀 认知受损的动物和相对 幸免 2)对所有猴子进行MCI和PET扫描， 灌注，使定量体积和代谢分析， 进行。3)对波士顿的猴子进行灌注 如果有必要的话，在耶基斯。 4)准备并分发适当的 从每只动物的大脑半球采集新鲜或冷冻组织样本， 的项目。 5)从大脑中连续切下整个半球切片， 另一个半球的所有动物，并准备四个档案 一系列染色切片（髓磷脂、硫堇、H&E和乙酰胆碱酯酶）， 所有的项目。 6)进行尸检并监测结果 对所有动物进行尸检，以确保没有隐性疾病 可能会混淆我们对年龄关系的观察 大脑中的变化与认知功能的变化有关。 7)到 维持健康记录和其他数据的综合数据库 摘自耶基斯动物记录系统，实验 操作进行的程序和结果变量的 不同的项目，并进行全面的统计分析， 这些变量，以确定主要因素有助于年龄相关的 认知功能障碍 8)鼓励并提供给其他人 研究人员从我们的动物脑组织和其他组织 这些都不是本项目的具体目标所需要的。

The Scientific Resource core is responsible for carrying out a variety of scientific procedures to provide both data and tissue samples to the various projects and to perform overall data management and statistical analysis. The specific functions of this core are as follows: 1) To assign animals in a balance fashion to the three different perfusion groups to ensure an equal distribution of age animals that are cognitively impaired and those that are relatively spared. 2) To conduct MCI and PET scans on all monkeys before perfusion to enable quantitative volumetric and metabolic analyses to be conducted. 3) To conduct all perfusions of monkeys both in Boston and if necessary at Yerkes. 4) To prepare and distribute appropriate fresh or frozen tissue samples from hemisphere of each animal to all of the projects. 5) To cut serial whole hemisphere sections from the other hemisphere of all of the animals and prepare four archival series of stained sections (myelin, thionin, H&E and AChE) for use by all the projects. 6) To conduct and monitor the results of postmortem autopsies on all animals to ensure that there are no occult diseases that could confound our observations on the relationship of age- related changes in the brain to changes in cognitive function. 7) To maintain an integrated database of health records and other data extract from the Yerkes Animal Records System, experimental manipulations conducted by the program and the outcome variables of the different projects and to perform overall statistical analyses of these variables to identify major factors contributing to age-related cognitive dysfunction. 8) To encourage and make available to other investigators both brain tissue and other tissues from our animals that are not needed for the specific aims of this project.