CELLULAR IMMUNITY TO CRYPTOSPORIDIUM PARVUM

描述(改编自申请者摘要)：调查员 建议检验CD40L与活化的T细胞结合的假设 感染上皮细胞表面CD40+有助于抗病毒免疫 胆道和肠道中的微小隐孢子虫(CP)。这个 研究人员建议研究CD40-CD40L 相互作用通过决定是否：1.CD40L参与CP免疫 是CP特异性免疫的传入或传出肢体所必需的 CP免疫和非免疫细胞向CP感染MHC的转移配型 淘汰赛获得者。2.γ-干扰素、肿瘤坏死因子受体和/或IL的完整基因 12是CP感染小鼠胆管硬化所必需的。3. 在细胞特异性的基础上，通过以下方式抑制Bc l系列蛋白的合成 CP感染。如果调查者证明CP可以从 非特异性和特异性T细胞介导的胆道树 一项针对艾滋病患者的后续临床研究，使用 将计划进行判别分析。

DESCRIPTION (Adapted from the Applicant's Abstract): The investigator proposes to test the hypothesis that binding of CD40L on activated T cells to CD40+ on infected epithelial cells contributes to immunity against Cryptosporidium parvum (Cp) in the biliary tree and the intestine. The investigator proposes to investigate the mechanisms by which CD40-CD40L interactions contribute to Cp immunity by determining whether: 1. CD40L is required for the afferent or efferent limb of Cp specific immunity by transfers of Cp immune and non-immune cells into Cp infected MHC matched knockout recipients. 2. The intact genes for gamma-IFN, TNFR, and/or IL 12 are required for sclerosis of bile ducts in Cp infected mice. 3. The synthesis of Bcl-series proteins is inhibited on a cell-specific basis by Cp infection. If the investigator shows that Cp can be eliminated from the biliary tree by non-specific as well as specific T-cell mediated responses, a subsequent clinical study in AIDS patients, using discriminant analysis, would be planned.