NATURAL HISTORY OF BABESIOSIS

巴贝斯虫病的自然史

• 批准号: 2887416
• 负责人: Peter Johnson Wettstein
• 金额: $ 26.63万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887416
• 关键词: SCID mouse; acute disease /disorder; babesiosis; cellular immunity; chronic disease /disorder; clinical chemistry; clinical research; comorbidity; disease /disorder model; disease /disorder proneness /risk; disease carrier state; emerging infectious disease; gene expression; genetic library; genetic strain; hamsters; histopathology; human subject; humoral immunity; laboratory mouse; model design /development; pathologic process; polymerase chain reaction; serology /serodiagnosis

The blood parasite Babesia microti and the spirochetal agent of Lyme disease, B. burgdorferi, are both transmitted by the deer tick, Ixodes dammini ( also called I. scapularis) and are transmitted in areas that are currently endemic for Lyme disease. In humans, infection with these two organisms may occur alone or in combination, and initial prospective studies suggest that persistence of infection with B. burgdorferi and the B. microti piroplasm may be enhanced by concurrent infection. Cryptic Babesia microti infection is of special concern to blood banks in endemic areas, since asymptomatic blood donors harboring infection with the protozoan have been implicated in many transfusion-acquired cases in this country, and one transfusion-acquired case has now occurred with the newly described Babesia-like piroplasm (WAI) in the western United States. Surprisingly little is known about the natural history of babesial infection. The specific aims enumerated here are oriented toward defining further the course of babesiosis in humans and in mouse models of acute and chronic infection that we are studying. First, since most piroplasm species studied to date comprise a chronic carrier state as part of their transmission cycle, we will attempt to document the chronic carrier state in humans, and to monitor the course of infection by using molecular and immunologic methods. Second, we animals and humans by constructing genetic expression libraries from piroplasm-specific genomic DNA, followed by immunologic screening of the libraries with reactive sera. This will help us to identify babesial proteins that are expressed in vivo and will define with greater precision the serologic responses during piroplasm infection. In the third aim, we will develop and evaluate mouse models of infection with a special emphasis on mouse strain susceptibility to piroplasm infection. Finally, in the fourth aim, we will evaluate the potential immunosuppressive properties of babesial infection in a mouse model of B. burgdorferi and B. microti coinfection. The information gained by these studies will lead ultimately to a better understanding of the natural history of babesial infection and the risks posed to humans infected with this emerging pathogen.

血液寄生虫田鼠巴氏杆菌和莱姆病螺旋体 疾病，B。burgdorferi，都是由鹿蜱，硬蜱传播的 dammini（也称为I.肩胛肌），并在 目前是莱姆病的地方病 在人类中，感染这两种病毒 微生物可能单独或组合发生， 研究表明持续感染B.布格多费里和 B。同时感染可使微粒体增强。 神秘 在流行性出血热中， 地区，因为无症状献血者携带感染 原生动物与许多输血获得性病例有关， 国家，一个输血获得的情况下，现在已经发生了新的 描述了美国西部的巴贝西亚样梨质体（阿威）。 令人惊讶的是，人们对巴贝斯尔的自然历史知之甚少 感染 这里列举的具体目标是为了确定 进一步的巴贝斯虫病在人类和小鼠急性和慢性感染模型中的过程， 我们正在研究的慢性感染。首先，由于大多数梨质体物种 迄今为止的研究包括慢性携带者状态作为其 传播周期，我们将尝试记录慢性携带状态 在人类中，并通过使用分子和 免疫学方法。 第二，我们动物和人类通过构建基因 来自梨质体特异性基因组DNA的表达文库，然后 用反应性血清对文库进行免疫学筛选。 这将有助于 我们确定巴贝斯虫蛋白，在体内表达，并将定义 更精确地检测梨浆菌感染期间的血清学反应。 在第三个目标中，我们将开发和评估感染的小鼠模型 特别强调小鼠品系对梨形物的易感性 感染 最后，在第四个目标中，我们将评估 巴贝斯虫感染小鼠模型B的免疫抑制特性 burgdorferi和B.田鼠混合感染。 通过这些获得的信息 这些研究最终将使人们更好地了解自然界 巴贝斯虫感染史和对感染巴贝斯虫的人构成的风险 这种新出现的病原体

项目成果

A polymorphic multigene family encoding an immunodominant protein from Babesia microti.

编码来自田鼠巴贝虫的免疫显性蛋白的多态性多基因家族。

• DOI: 10.1128/jcm.38.1.362-368.2000
• 发表时间: 2000
• 期刊: Journal of clinical microbiology
• 影响因子: 9.4
• 作者: Homer,MJ;Bruinsma,ES;Lodes,MJ;Moro,MH;Telford3rd,S;Krause,PJ;Reynolds,LD;Mohamath,R;Benson,DR;Houghton,RL;Reed,SG;Persing,DH
• 通讯作者: Persing,DH