DEFINING HEPATITIS C VIRUS-SPECIFIC CTL ACTIVITY IN MAN

定义人类丙型肝炎病毒特异性 CTL 活性

• 批准号: 2887430
• 负责人: K J Kao
• 金额: $ 18.73万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887430
• 关键词: cell line; chronic disease /disorder; clinical research; cytotoxic T lymphocyte; cytotoxicity; diagnosis design /evaluation; epidemiology; epitope mapping; hepatitis C; hepatitis C virus; human subject; peptide library; phenotype; tissue /cell culture; transfection; vaccinia virus; virus genetics

DESCRIPTION: This proposal has two major specific aims: 1) To demonstrate the existence HCV-specific liver-infiltrating cytotoxic T lymphocytes in chronic HCV infection. The applicant has already determined the HCV genotype of many patients and will complete this. To detect the presence of HCV-specific CTLs, he will employ a target cell consisting EBV immortalized B-lymphoblastoid cells from each patient with chronic HCV. These cells will be infected with vaccinia virus carrying HCV gene inserts coding for specific candidate regions that are suspected of serving as epitopes in the CTL recognition process. CTLs will be obtained by bulk expansion of lymphocytes harvested from liver biopsies. chromium release assay of target cell lysis will be used to detect cells and quantitate the cytotoxic activity. 2) The second specific aim is to define the actual epitopes involved in the CTL- mediated recognition. CTLs identified in specific aim 1 will be cloned for use in screening small synthetically produced peptides overlapping in regions identified in specific aim 1. Sensitization of target cells and the cytolysis assay will be used.

描述：本提案有两个主要的具体目标：1)

项目成果

Pathogenesis of chronic hepatitis C virus infection.

慢性丙型肝炎病毒感染的发病机制。

• 发表时间: 1998
• 期刊: Antiviral therapy.
• 作者: Nelson,DR;Lau,JY
• 通讯作者: Lau,JY

Clinical, virological and histological implications of GB virus-C/hepatitis G virus infection in patients with chronic hepatitis C virus infection: a multicentre study based on 671 patients.

GB 病毒-C/肝炎病毒感染对慢性丙型肝炎病毒感染患者的临床、病毒学和组织学影响：一项基于 671 名患者的多中心研究。

• DOI: 10.1046/j.1365-2893.2000.00188.x
• 发表时间: 2000
• 期刊: Journal of viral hepatitis.
• 作者: Slimane,SB;Albrecht,JK;Fang,JW;Goodman,Z;Mizokami,M;Qian,K;Lau,JY
• 通讯作者: Lau,JY

High prevalence of GB virus-C/hepatitis G virus infection in liver transplant recipients.

肝移植受者中 GB 病毒-C/庚型肝炎病毒感染的患病率较高。

• DOI: 10.1097/00007890-199706150-00028
• 发表时间: 1997
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: Dickson,RC;Qian,KP;Lau,JY
• 通讯作者: Lau,JY

Hepatic expression of cell proliferation markers and growth factors in giant cell hepatitis: implications for the pathogenetic mechanisms involved.

巨细胞肝炎中细胞增殖标志物和生长因子的肝脏表达：对相关发病机制的影响。

• DOI: 10.1097/mpg.0b013e3181f85a87
• 发表时间: 2011
• 期刊: Journal of pediatric gastroenterology and nutrition
• 影响因子: 2.9
• 作者: Fang,JaneWS;Gonzalez-Peralta,ReginoP;Chong,SonnyKF;Lau,GraceM;Lau,GillianM;Lau,JohnsonYN
• 通讯作者: Lau,JohnsonYN

Mechanisms for genetically predetermined differential quantitative expression of HLA-A and -B antigens.

• DOI: 10.1016/s0198-8859(00)00142-7
• 发表时间: 2000-08
• 期刊: Human immunology
• 影响因子: 2.7
• 作者: K. Liu;K. Kao